Protein Info for Pf1N1B4_5102 in Pseudomonas fluorescens FW300-N1B4

Annotation: Putative glutathione transporter, permease component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 315 transmembrane" amino acids 14 to 34 (21 residues), see Phobius details amino acids 103 to 124 (22 residues), see Phobius details amino acids 136 to 160 (25 residues), see Phobius details amino acids 179 to 198 (20 residues), see Phobius details amino acids 239 to 265 (27 residues), see Phobius details amino acids 283 to 308 (26 residues), see Phobius details PF19300: BPD_transp_1_N" amino acids 6 to 80 (75 residues), 55.3 bits, see alignment E=7.4e-19 PF00528: BPD_transp_1" amino acids 117 to 313 (197 residues), 148 bits, see alignment E=2.6e-47

Best Hits

Swiss-Prot: 36% identical to GSIC_ECOL6: Glutathione transport system permease protein GsiC (gsiC) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K02033, peptide/nickel transport system permease protein (inferred from 91% identity to pba:PSEBR_a716)

MetaCyc: 35% identical to glutathione ABC transporter membrane subunit GsiC (Escherichia coli K-12 substr. MG1655)
RXN0-11 [EC: 7.4.2.10]

Predicted SEED Role

"Putative glutathione transporter, permease component" in subsystem Utilization of glutathione as a sulphur source

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QFL1 at UniProt or InterPro

Protein Sequence (315 amino acids)

>Pf1N1B4_5102 Putative glutathione transporter, permease component (Pseudomonas fluorescens FW300-N1B4)
MNLSRYRFILSRPLQLLPVLFGISLITFVLVRSIPGDPARALLGSRSTPEGLLRVRAQFG
LDQPLWMQYFYFLKNLFNGDLGQSLLYKVDALKLISTRIEPTLFLVLGSVLLAMLIAVPL
ATVAARNKGGWGDNLIRLFTTAGLGMPAFWLGIMLILLFSVQLGWFPVSGYGRSWPDKLH
HMVLPCLTIALALSAVLIRNLRASMLMELQADHVTAARARGLPENVVFRRHVLPNSLVPA
VNLLAVNIGWLISGTVVIESLFAIPGIGQLLVRGIFTRDYMVVQGVAMVLACATVAVNFL
ADVVTVAIDPRVKIR