Protein Info for Pf1N1B4_5096 in Pseudomonas fluorescens FW300-N1B4

Annotation: Nitrate ABC transporter, nitrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 transmembrane" amino acids 23 to 42 (20 residues), see Phobius details PF13379: NMT1_2" amino acids 53 to 303 (251 residues), 342.2 bits, see alignment E=1.2e-106

Best Hits

KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 81% identity to pmy:Pmen_2105)

Predicted SEED Role

"Nitrate ABC transporter, nitrate-binding protein" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QFH9 at UniProt or InterPro

Protein Sequence (428 amino acids)

>Pf1N1B4_5096 Nitrate ABC transporter, nitrate-binding protein (Pseudomonas fluorescens FW300-N1B4)
MTDERDSQHPVHPGRRTFLKQSLVTAGTVAGIAALDVVVPGFLRSAVWAAGSDAPEKAAL
KVGFIPLTDCASVVVAATQGFGEKYGLTITPSKEASWAGVRDKLLNGELDAAHVLYGLIY
GVQLGIGGPQKDMAMLMGLNQNGQAITLSNQLQQAGVTNGEQLAAKVKQGGTPLTFAQTF
PTGTHAMWLNYWLASLGINPLTDVKTIVVPPPQMVANMRVGNMDGFCVGEPWGARAIADN
IGFTALTTQKLWPDHPEKILGTTRAFVEQYPNTARALTMAVLDASRFIEASVENKKSTAQ
LIAGKAYVNAPVEVIEQRFLGHYDDGLGKQWTDEHAMSFCKDGSVNYPYLSDGMWFLTQF
RRWGLLKEDVDYAGVAKSINQTKLYGEAASQLGLTVPGSPLRSSTLIDGKVWDGSNPAAY
ANSFTIKA