Protein Info for Pf1N1B4_5092 in Pseudomonas fluorescens FW300-N1B4

Annotation: UDP-glucose 4-epimerase (EC 5.1.3.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 3 to 115 (113 residues), 31.7 bits, E=4.2e-11
• PF08659: KR
  • amino acids 4 to 136 (133 residues), 36 bits, E=2.8e-12
• PF04321: RmlD_sub_bind
  • amino acids 5 to 164 (160 residues), 43.4 bits, E=9.6e-15
• TIGR01179: UDP-glucose 4-epimerase GalE
  • amino acids 6 to 334 (329 residues), 436.2 bits, E=3.2e-135
• PF02719: Polysacc_synt_2
  • amino acids 6 to 186 (181 residues), 54.9 bits, E=3.1e-18
• PF01073: 3Beta_HSD
  • amino acids 6 to 132 (127 residues), 52.9 bits, E=1.1e-17
• PF16363: GDP_Man_Dehyd
  • amino acids 6 to 325 (320 residues), 171.6 bits, E=1.3e-53
• PF01370: Epimerase
  • amino acids 6 to 264 (259 residues), 190.4 bits, E=1.5e-59
• PF07993: NAD_binding_4
  • amino acids 7 to 184 (178 residues), 26.7 bits, E=1.3e-09

Best Hits

Swiss-Prot: 57% identical to GALE_YEREN: UDP-glucose 4-epimerase (galE) from Yersinia enterocolitica

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 77% identity to pfo:Pfl01_2824)

MetaCyc: 55% identical to UDP-glucose 4-epimerase (Bacillus subtilis subtilis 168)
UDP-N-acetylglucosamine 4-epimerase. [EC: 5.1.3.7]; UDP-glucose 4-epimerase. [EC: 5.1.3.7, 5.1.3.2]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (9/11 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (2/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2 or 5.1.3.7

Sequence Analysis Tools

See A0A166QFC1 at UniProt or InterPro

Protein Sequence (354 amino acids)

>Pf1N1B4_5092 UDP-glucose 4-epimerase (EC 5.1.3.2) (Pseudomonas fluorescens FW300-N1B4)
MHKTTLITGGAGYIGSHTTLTLIDAGRQVLVLDNLCNSCHESLTRLEYLTRKRVDFIEGD
IRDSHLLDDIFSRYDIDAVVHFAGLKSVEESVRKPLDYYANNVVGTLNLCHAMARFEVFK
LVFSSSATVYGAPRQIPLIEDMGAGKPINPYGRTKLLIEELLTDLCLSDPQWSIAVLRYF
NPIGAHASGLIGENPNGRPNNLLPCLTRVAMRQVPELMVYGSDYPTVDGTCVRDYVHVLD
VADGHLKALRALQNTNGIHVWNLGTGIGHSVLQIIHSFENITGITIPFRFAPRRDGDIAE
CWADPTKAGRDLGWYAQRDLMQMIIDTWRWQLCNPYGYHSQPGLAASTALKQWI