Protein Info for Pf1N1B4_5076 in Pseudomonas fluorescens FW300-N1B4

Annotation: FIG00641944: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 PF07005: SBD_N" amino acids 10 to 236 (227 residues), 268.1 bits, see alignment E=7.7e-84 PF17042: NBD_C" amino acids 263 to 418 (156 residues), 145.4 bits, see alignment E=2.4e-46

Best Hits

Swiss-Prot: 86% identical to OTNK_PSEF5: 3-oxo-tetronate kinase (otnK) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: None (inferred from 87% identity to pfs:PFLU2353)

MetaCyc: 73% identical to 3-dehydrotetronate 4-kinase monomer (Cupriavidus necator H16)
RXN-18594 [EC: 2.7.1.217]; 2.7.1.217 [EC: 2.7.1.217]

Predicted SEED Role

"FIG00641944: hypothetical protein"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.217

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QEY6 at UniProt or InterPro

Protein Sequence (430 amino acids)

>Pf1N1B4_5076 FIG00641944: hypothetical protein (Pseudomonas fluorescens FW300-N1B4)
MNTTTARPLLGCIADDFTGATDLANMLVRGGMRTVQSIGIPSSEVAAGLDADAIVIALKS
RTIPVAEAVEQSLAALAWLREQGCEQIFFKYCSTFDSTAAGNIGQVSEALLAALGSDFTL
ACPAFPENGRTIFRGHLFVQDQLLNESGMQHHPLTPMTDANLVRVLQSQTGLNVGLLRYD
TIAQGAEQVRERIAELRAQGVGMAIADALSDSDLYTLGTACADLPLLTGGSGLALGLPEN
FRRAGKLRDLDASKLLAVSGGEVVLAGSASIATNGQVAAWLEAGRPALRIDPLALAAGEP
VVAQALAFARDNEQTVLIYATSTPDEVKAVQQQLGVERAGTLVENALGEIAQGLRQSGVR
RFVIAGGETSGAVVQALGVQLLQIGAQIDPGVPATVSSTDEPLALALKSGNFGGRDFFNK
ALKQLAGGAQ