Protein Info for Pf1N1B4_5057 in Pseudomonas fluorescens FW300-N1B4
Annotation: mandelate racemase family protein Pfl_3283
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01706, glucarate dehydratase [EC: 4.2.1.40] (inferred from 93% identity to pba:PSEBR_a3183)Predicted SEED Role
No annotation
MetaCyc Pathways
- D-glucarate degradation II (3/3 steps found)
- superpathway of D-glucarate and D-galactarate degradation (4/5 steps found)
- D-glucarate degradation I (3/4 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (14/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.40
Use Curated BLAST to search for 4.2.1.40
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A162B0Z5 at UniProt or InterPro
Protein Sequence (424 amino acids)
>Pf1N1B4_5057 mandelate racemase family protein Pfl_3283 (Pseudomonas fluorescens FW300-N1B4) LKIKRVTVTPIAFRDPPLLNASGIHEPFALRSIIEIESDNGYIGLGESYGDAPALAIQQQ LQSQLIGLDPFNLNQLRAIVQATVAANKTQGIAGAELAPGSHASKAVSNAYSAFEVAFLD LQAHSLNVPLVDLLGGAIRDEVPFSAYLFFKYAQHIDSPYKPDSWGEALNEEQIVAQARR MIEAYGFKSIKLKAGALPPEHEVSCIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD DLQYYEDPTPGLDGMAELHKRTGLPLATNMVVTDFDEFRRSVALNSVQIVLADHHYWGGL RDTQALAKMCDTFGLGVSMHSNSHLGISLMAMAHVAASVPNLDYACDTHYPWQEPDEEVI KGGKLSIVDGCVKITRAPGLGLELDHDQLGKLHDQYLSCGIRQRDDVKQMQRYKPEWKTI KPRF