Protein Info for Pf1N1B4_495 in Pseudomonas fluorescens FW300-N1B4

Annotation: Flp pilus assembly protein TadB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 transmembrane" amino acids 6 to 33 (28 residues), see Phobius details amino acids 99 to 118 (20 residues), see Phobius details amino acids 124 to 140 (17 residues), see Phobius details amino acids 269 to 289 (21 residues), see Phobius details amino acids 301 to 320 (20 residues), see Phobius details PF19360: TadB_TadC_N" amino acids 11 to 66 (56 residues), 27.8 bits, see alignment E=1.9e-10 PF00482: T2SSF" amino acids 161 to 286 (126 residues), 77.6 bits, see alignment E=8.2e-26

Best Hits

KEGG orthology group: K12510, tight adherence protein B (inferred from 88% identity to pba:PSEBR_a4086)

Predicted SEED Role

"Flp pilus assembly protein TadB" in subsystem Widespread colonization island

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MNL6 at UniProt or InterPro

Protein Sequence (328 amino acids)

>Pf1N1B4_495 Flp pilus assembly protein TadB (Pseudomonas fluorescens FW300-N1B4)
MNNIPGEFILIFLGMVFIAVFLLSQGVVVPVFGEAGKMRKRIRGRLNVLEKANNLPNMQT
VLRQKYLTRLSPLEAMLEQLPFMASLTQVIEQGGHEYRAYRVLLLGLFLGVCAAISIWMI
SAVWWMALLAGFGVFWLPLLKISRDRGKRFAEFEEGLPDALDAMCRALRAGHPFNETLRL
VAEEHKGAVAHEFGLTFSDINYGNDVRRAMLGLLERMPSMTVMMLVTSILIHRETGGNLT
EVLERLSRLIRGRYRFQRKIKTLSAEGRMSAWILVAIPFVLAIAIIVTSPTYMPVLLNDP
IGHKLIIGAFCAMLIGIFWIKKIIRIQV