Protein Info for Pf1N1B4_4912 in Pseudomonas fluorescens FW300-N1B4

Annotation: Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 162 PF13412: HTH_24" amino acids 7 to 53 (47 residues), 65.9 bits, see alignment E=2.9e-22 PF13404: HTH_AsnC-type" amino acids 7 to 48 (42 residues), 60.3 bits, see alignment E=1.8e-20 PF01037: AsnC_trans_reg" amino acids 74 to 146 (73 residues), 65.6 bits, see alignment E=4.4e-22

Best Hits

Swiss-Prot: 46% identical to DOEX_HALED: Transcriptional regulatory protein DoeX (doeX) from Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)

KEGG orthology group: None (inferred from 99% identity to pfs:PFLU4034)

Predicted SEED Role

"Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system" in subsystem Branched-Chain Amino Acid Biosynthesis or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H124 at UniProt or InterPro

Protein Sequence (162 amino acids)

>Pf1N1B4_4912 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system (Pseudomonas fluorescens FW300-N1B4)
MEGLVKLDRIDISILVELQKDGRMTNVSLADAVGLSASPCLQRVKRLESAGYISSYKAHL
NLAKITDSVTVFTEITLSDHKREDFAKFESNIRLVDEVLECHLISGGYDYLVRFMTRSIQ
HYQEVIESLLDKNIGISKYFSYIVIKSPVLKDGVPLRKLLRH