Protein Info for Pf1N1B4_4906 in Pseudomonas fluorescens FW300-N1B4

Annotation: Lipase (EC 3.1.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 566 PF00353: HemolysinCabind" amino acids 320 to 347 (28 residues), 30.2 bits, see alignment (E = 1.7e-11) amino acids 341 to 366 (26 residues), 9.3 bits, see alignment (E = 6.3e-05) amino acids 441 to 468 (28 residues), 12.8 bits, see alignment (E = 5.1e-06) amino acids 474 to 489 (16 residues), 9.5 bits, see alignment (E = 5.2e-05)

Best Hits

KEGG orthology group: K01046, triacylglycerol lipase [EC: 3.1.1.3] (inferred from 84% identity to pfo:Pfl01_2685)

Predicted SEED Role

"Lipase (EC 3.1.1.3)" (EC 3.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.3

Use Curated BLAST to search for 3.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QBB1 at UniProt or InterPro

Protein Sequence (566 amino acids)

>Pf1N1B4_4906 Lipase (EC 3.1.1.3) (Pseudomonas fluorescens FW300-N1B4)
MGLFDYKNAGSEAGKALYSDAIALTLYAYTPTGQPLPATAWAPIGAAALGYQGNVGAQGT
FYGEKDGFTSAEAEVLGKYDGAGKLIGIGIAFRGTGGLGYSDTFGDMKNNLLAAVGPVDY
ATNYAKNAFDTLLKSVAAFAIAHGLSAKDVLVSGHSLGGLGVNSLAELSGNNWGGFFKDA
NYIAFASPTQSATGNNVLNIGYENDPVFRVLDGTTFSSGSLGKHDGHQESATNNIVNFND
QYASTAQNLVPFSILNPLNWSAHGSLGYADGLNRVIDSRFYDLTDKDSTLIVSNLSESSR
GSTWVEDLGRSGEPHTGSTFIIGTDSNDWLKGGAGNDFIEGRDGNDRFRDDGGYNLLLGG
KGSNTFELQKPLQNFSFANDGDGTLYVRDAYGGISMTRDIGALVSKESGSWWGSKEVTYN
VTANGLLNGTELTHYNHSLNGDAYGNTLAATLDGDWLFGNAGDDLLRSDKSHVTFVGGAG
NDVMQASGGGNNTFLFSGAFGFDAINGYQGSDKLVFMGVQGAGQGYDYTQHATQAGNDTV
LKIGDYAVTLVGVGLDNLSASGITFA