Protein Info for Pf1N1B4_4904 in Pseudomonas fluorescens FW300-N1B4

Annotation: Serine protease homologue

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 987 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF00082: Peptidase_S8" amino acids 68 to 357 (290 residues), 147.3 bits, see alignment E=9e-47 TIGR02601: autotransporter-associated beta strand repeat" amino acids 453 to 484 (32 residues), 40.6 bits, see alignment (E = 1.6e-14) PF12951: PATR" amino acids 454 to 483 (30 residues), 43.6 bits, see alignment (E = 3.5e-15) TIGR01414: outer membrane autotransporter barrel domain" amino acids 518 to 987 (470 residues), 168.1 bits, see alignment E=3.5e-53 PF03797: Autotransporter" amino acids 716 to 967 (252 residues), 215.2 bits, see alignment E=2.1e-67

Best Hits

KEGG orthology group: K12685, subtilase-type serine protease [EC: 3.4.21.-] (inferred from 84% identity to pfo:Pfl01_2683)

Predicted SEED Role

"Serine protease homologue"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161ZET2 at UniProt or InterPro

Protein Sequence (987 amino acids)

>Pf1N1B4_4904 Serine protease homologue (Pseudomonas fluorescens FW300-N1B4)
MHMDVQIKPMSVGTLLLLISSVPGPAQALYLETGKPGDAASWRSTEFQRDWGLARMQADQ
AYAAGITGKGVKIGALDSGFDSSHPEFAADRYHAVTASGSYVDGSAFNVDGTLNTNNDSH
GTHVTGTMGASRDGTGMHGVAYNAQIYVGNTNKNDSFLFGPNPDPRYFKAVYNALADAGV
RAINNSWGSQPPDVNYRTLDDLHAAYAQHWNKGTWLDAAADVSRRGVINVFSAGNSGYPN
ASVRSALPYFQPDLEGHWLAVSGLDQSNQQKYNQCGIAKYWCITTPGAKIDSTIPGGGYA
IKSGTSMAAPHATGALALVMERYPYMNNQQALEVLLTTATQLDGSITEAPSTRIGWGVAN
LERAMRGPGQLLGSFDANLGAGQSDVWSNDISDKALIQRQSEDAAERSAWQQTLKDKGWE
NGIAAGASQQDQTDYAVGTARDSAAANRVYEGSLIKSGAGRLMLTGDNTYRGPTTVNGGL
LAVNGSLTSAVTVNDSGTLGGNGRIAALTANAGGTVAPGNSIGTLQVSGDVTFAPGSTYA
VELSPTSSDQIITSGTATISGATVSLSLENSPTLLSTQQVQSLLGHQYNILQAAGGIQGQ
FGAVLPNYLFIGGSLDYAVTGIQLSVERNATTFASVGQTPNQRNVAAAVEGLGAGNAVYE
SLLLSPTASSAQQAFQQLSGEIYPALGSVLINDSRYLRDAVGERLNEASGSRSNGWIKAL
GAWGKTDDSHDTAGYTTSIGGLLAGVDGAVDDETRVGLVAGYSDSSASMGSGTHSSGQVD
SYHLGAYAGHEIGAWRLSAGGAYSRHRADVKRDLQYGDVSAKQKAKVDAGTTQLFGEAAY
RLNLQALALEPFANLAYVHLDTEGFTEKGDAAALKNSGDRRDAVLSTLGVRALKTVTLSG
QQQLDLSGSLGWQHNLSNTDSEEHLAFASGGTPFVVQSSPLVRDAALVGAHASLALSRDV
RLNLDYTGQLANRERSHGVGLSLNWQF