Protein Info for Pf1N1B4_4893 in Pseudomonas fluorescens FW300-N1B4
Annotation: O-acetylhomoserine sulfhydrylase (EC 2.5.1.49)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 86% identity to pfo:Pfl01_2675)Predicted SEED Role
"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49)" in subsystem Methionine Biosynthesis (EC 2.5.1.49)
MetaCyc Pathways
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
- L-methionine biosynthesis III (4/4 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
- L-homocysteine biosynthesis (2/2 steps found)
- superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
- S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)
- S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (2/5 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.49
Use Curated BLAST to search for 2.5.1.49
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A161ZDL2 at UniProt or InterPro
Protein Sequence (468 amino acids)
>Pf1N1B4_4893 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) (Pseudomonas fluorescens FW300-N1B4) MSAWRTISLWMDQLDEPLLARPALEQDLDVDVAIIGAGYTGLWAAYYLKRLAPELDIAIV EAQTAGFGASGRNGGWLMGNLLGEDRLLADLPAEQRRASFELLHGIPDEVAIVLEREGID CDYRKGGVLYCAARYPEQEASLRHYLDTLYHQGLTESDYRWLSPEQLAQQIRVAKPYGGI YAPHVATIHPAKLVRGLARTVERMGVNIYENSPVTQWQSGSLRTAKAQVRSRWIVPALEG YAATLPPLDRYQLPVQSLLVATEPLSAATWDEIGLSHGQAFSESSRQVTYGQRTADNRLV FGARGGYQFAGKLRHDFDLTTSEIELRRYLFSELFPQLKNVRITHAWGGNLGMSRRFKPH MLCDQASGIALSGGYGGEGVGASNLGGRTLADLILQRDTDLVRQPWVIPQGGLDALKAWE PEPCRWLGYNAIIRSFVHEDQTLANPATAPWRRKLASRVAGFMEGFMH