Protein Info for Pf1N1B4_4869 in Pseudomonas fluorescens FW300-N1B4

Annotation: Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 746 transmembrane" amino acids 34 to 53 (20 residues), see Phobius details PF20256: MoCoBD_2" amino acids 68 to 207 (140 residues), 45.2 bits, see alignment E=9.1e-16 amino acids 618 to 698 (81 residues), 37.5 bits, see alignment E=2.1e-13 PF02738: MoCoBD_1" amino acids 348 to 575 (228 residues), 102.1 bits, see alignment E=2.7e-33

Best Hits

KEGG orthology group: K07303, isoquinoline 1-oxidoreductase, beta subunit [EC: 1.3.99.16] (inferred from 86% identity to pba:PSEBR_a2854)

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QAN6 at UniProt or InterPro

Protein Sequence (746 amino acids)

>Pf1N1B4_4869 Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16) (Pseudomonas fluorescens FW300-N1B4)
MFNDTYPDELPPVLQHILERDQIDGPTTLPRRSFLKILGVGGLALGAFPHLALAQQPGDN
RAMPLKPSQQPSAFVQIAPNGEVTVTINRLEFGQGVQTGLPMILAEELDADWSLVRSRNG
TSDIAYQDPLFGMHLTGGSNSIKNSYTQYRELGARARAMLLAAAAARWNVDMASLSTQSG
MVLGPAGRKASYGELAEAAMALPVPETITLKDPKDFRIIGHATTRIDAKAKSSGQQDFGI
DMKLPGQLTAVVARPPVFGAKIASLDDSAARAIKGVKAVLRVPLDGGAEGVAVVADGYWQ
AKQGRDALKLQWDTTTVEKVDSDKQLADYRELAGRPGPRLFEADMAPLETAPHRLDAEFV
FPYLAHAPMEPLNCTVKLSEERCELWVGTQFAGADSAAAARVLNLKPEQIQINVQTAGGG
FGRRGIATSDIVVLACEVAKAARMAGLNVPVRTLWSREDDIKGGYYRPMHLHRARLGFDD
TGKVLAWDHVVVGQSIISGTMFGGRVKNGIDPTATEGMRDPYPLPMRLTVHHPKLNVPVL
WWRSVGSTHTAFVMETVIDEIARTTKQDPVAYRMKLFADQSPRHRAALQLAVDKSEYGKR
ELPAGRAWGVAVHESFSSVVAYVVEASVQNGRPVLHHVTAGVHCNLVVNPRSVEAQVQGA
ALMGLSMCLPGGAITLKDGVVQQSNFADFSVPRITDMPEFSIHVVPSAEPPTGMGEPGLP
PLAPAFANAVARLTGKPVRELPFKLT