Protein Info for Pf1N1B4_4856 in Pseudomonas fluorescens FW300-N1B4

Annotation: Signal transduction histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1163 transmembrane" amino acids 20 to 44 (25 residues), see Phobius details amino acids 184 to 206 (23 residues), see Phobius details PF05227: CHASE3" amino acids 46 to 179 (134 residues), 134.6 bits, see alignment E=7e-43 PF13185: GAF_2" amino acids 252 to 388 (137 residues), 62 bits, see alignment E=2.2e-20 PF01590: GAF" amino acids 256 to 387 (132 residues), 39.8 bits, see alignment E=1.9e-13 PF00512: HisKA" amino acids 494 to 561 (68 residues), 63.7 bits, see alignment 4e-21 PF02518: HATPase_c" amino acids 609 to 718 (110 residues), 102.4 bits, see alignment E=6.2e-33 PF00072: Response_reg" amino acids 773 to 879 (107 residues), 59.7 bits, see alignment E=9e-20 amino acids 895 to 1003 (109 residues), 52.4 bits, see alignment E=1.7e-17 amino acids 1041 to 1153 (113 residues), 93.9 bits, see alignment E=2.2e-30

Best Hits

KEGG orthology group: K00936, [EC: 2.7.3.-] (inferred from 89% identity to pfo:Pfl01_2646)

Predicted SEED Role

"Signal transduction histidine kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161ZDI2 at UniProt or InterPro

Protein Sequence (1163 amino acids)

>Pf1N1B4_4856 Signal transduction histidine kinase (Pseudomonas fluorescens FW300-N1B4)
MTSASSVDEQRFRKLLSRNVSLPLGVGVISAVFFVSLITYLLSVIQWVQHTDRVINNANE
AIKLTVDLETGMRGFLLSGDEHFLDPYETAKPRIAVALNTLLELTADNPVQTDRLRRLQA
LQTEWANYAQSMIDLQRASGDYRGVVKAGRGKRLTDEIRKQFEDVIDLEQQLRATRNEEV
RRTTIWSIALYLLFVAAISGLLAYIGRRDLLKLSQSYSTNLAGQQASARRLEQQAWLRNG
QTELAEQVLGQLSLNVLGRNILQFCAQYLGTAVAAIYVREDHGGLKRIASYGFSREQEAL
EQLIYSGEGIVGQVAQQARLIRLDNVPGDYFKVSSGLGEGLPHSVLVVPTSDDDRVNGVI
ELGFLRTLSDRDVELLELIAGNIGTSIEAARYRQRLQEVLAEAQQLNEELQVQQEELKTA
NEELEEQSRILKESQTHLETQQVALEQTNEQLAEQGQILAAQRDAMDLKNTELNLAQVEL
EARAEELQRSSKYKSEFLANMSHELRTPLNSSLILAKLLAENPQENLSAEQVKFAESIYS
AGNDLLNLINDILDISKVEAGKLEVRPENTSVARLVEGLRGMFQPLAAQKNLDLQIQLQA
DAPLMLLTDRQRLEQVIKNLLSNAVKFTEKGTVSLSVAAQPGEGIAFIIRDSGIGIAPDQ
QESIFEAFRQADGTINRRYGGTGLGLSISRDLATLLGGSISVTSTPGQGSVFTLVLPQQY
VEPDDGPVDAMEATPAVMPQSAMVAAPGPLIAAVDIPRFDDDRNKAPFATRCILVVEDEP
NFAHILYDLAHELGYQCLVAHGADEGYDLAMQFLPDAILLDMRLPDHSGLTVLQRLKEHA
ETRHIPVHVISVEDRVEAAMHMGAIGYAVKPTTREELKDVFARLEAKLTQKVKRVLLVED
DDVQRDSIARLIGDEDIEITAVGLAQDALDLLRTTIFDCMIIDLKLPDMLGNDLLKRMST
EDICSFPPVIVYTGRNLTRDEETELRKYSRSIIIKGARSPERLLDEVTLFLHKVESRLSQ
ERQTMLKIARSRDKVFEGRKVLLVDDDVRNIFALTSALEQKGAVVVIGRTGREAIEKLNE
VEDIDLVLMDVMMPEMDGFEATLEIRKDPRWRKLPIIAVTAKAMKDDQERCLQAGSNDYL
AKPIDLDRLFSLIRVWLPKMERI