Protein Info for Pf1N1B4_4825 in Pseudomonas fluorescens FW300-N1B4

Annotation: Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 362 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF13416: SBP_bac_8" amino acids 40 to 327 (288 residues), 117.8 bits, see alignment E=1.6e-37 PF01547: SBP_bac_1" amino acids 41 to 295 (255 residues), 57.7 bits, see alignment E=4.1e-19 PF13343: SBP_bac_6" amino acids 74 to 313 (240 residues), 44.5 bits, see alignment E=2.8e-15

Best Hits

Swiss-Prot: 64% identical to SPUE_PSEAE: Spermidine-binding periplasmic protein SpuE (spuE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02055, putative spermidine/putrescine transport system substrate-binding protein (inferred from 90% identity to pfo:Pfl01_2829)

MetaCyc: 55% identical to putrescine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166Q9Q0 at UniProt or InterPro

Protein Sequence (362 amino acids)

>Pf1N1B4_4825 Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2) (Pseudomonas fluorescens FW300-N1B4)
MRLLKSVVPASLALLFSVTAHAQSSVSVYNWTDYIGETTLADFQAKTGTKVIYDVFDSNE
TLEGKLLAGRTGYDVVVPSNHFLARQVKAGAFLKLDRSQLPNWKNLDPKLLALLEKSDPG
NEHSVPYLWGTNGIGYNVDKVKQVLGIDRIDSWAVFFEPENLKKLSQCGVSMMDSADEVF
PAVLNYMGMDPRSENPEDFKKAEAKLLTIRPYITYFHSSKYVSDLANGDICVAFGYSGDV
FQAANRAKEAKNGVNIAYAIPKEGSNLWFDLLAIPADASNPKEAHSFVNNLLDPQVIAKV
SASVGYANPNPAAKQYMDPELVNNPEVYPPQEVLDKLYISTTPPQPIMRLMTRSWSKVKS
NQ