Protein Info for Pf1N1B4_4772 in Pseudomonas fluorescens FW300-N1B4

Annotation: RND efflux system, inner membrane transporter CmeB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1032 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 339 to 358 (20 residues), see Phobius details amino acids 365 to 387 (23 residues), see Phobius details amino acids 393 to 413 (21 residues), see Phobius details amino acids 437 to 457 (21 residues), see Phobius details amino acids 472 to 496 (25 residues), see Phobius details amino acids 540 to 557 (18 residues), see Phobius details amino acids 867 to 886 (20 residues), see Phobius details amino acids 894 to 913 (20 residues), see Phobius details amino acids 920 to 942 (23 residues), see Phobius details amino acids 968 to 988 (21 residues), see Phobius details amino acids 999 to 1022 (24 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1021 (1021 residues), 1217.1 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1030 (1030 residues), 1526.5 bits, see alignment E=0 PF03176: MMPL" amino acids 342 to 511 (170 residues), 35.5 bits, see alignment E=5.9e-13

Best Hits

Swiss-Prot: 51% identical to TTGH_PSEPT: Toluene efflux pump membrane transporter TtgH (ttgH) from Pseudomonas putida (strain DOT-T1E)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 63% identity to atu:Atu3202)

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166Q8J6 at UniProt or InterPro

Protein Sequence (1032 amino acids)

>Pf1N1B4_4772 RND efflux system, inner membrane transporter CmeB (Pseudomonas fluorescens FW300-N1B4)
MPQFFIDRPVFAWVIALFILLAGALAIVQLPVAQYPNVAPPKVEIYATYPGASAQTVDES
VVSLIEEELNGADHLLYFESQSSLGSATITATFQPGTDPEMAQVDVQNRLKVVESRLPQA
VTQQGLQVEKVSAGFLLLITLTSSDGKLDDVALSDYLARNVMNEIKRLDGVGKAQLYGAE
RAMRIWIDPRKLIGFNLTPADVNAAIVAQNAQVSAGSIGDLPTRTTQEITATILVKGQLS
TPEEFADIVLKADPDGSTVRIGDVARVEIGSQEYQFSTRLNGKPSTAVSVQLSPGANALS
TATLVRAKMDELARYFPAGVEYKIPYDTSPFVKVSITKVVYTLIEAMVLVFAVMFLFLQN
IRYTLIPTLVVPVALMGTFATMLALGFSINVLTMFGMVLAIGILVDDAIVVVENVERIMA
TEGLSPKEATRKAMKQITGAIIGITLVLVAVFIPMAFMQGSVGVIYQQFSLSMATSILFS
AFLALTLTPALCATLLKPIAKGEHHAKGGFFGWFNRRFEQLTERYQGWVAYALKRTGRYL
LIYGVLLIGLGLCFSRLPSSFLPVEDQGYTITDIQLPPGASKNRTVQVVEQIEAHNATEP
GVGDSTVILGFSFSGSGQNAALAFTTLKDWSDRGSDDSASSIADRANIALSQIKDAMAFA
VLPPPVDGLGTSSGFEFRLQDRGGLGHATLMQARSQLLAAAEKSPVLMNVRESALAEAPQ
VQLEVDRKQANALGISFADIGNVLSTAVGSAYINDFPNQGRMQRVVVQAEGDQRSQVDDL
LKIHVRNNGENMVPLSAFVRANWTQGPAQLTRYNGYPAISISGEPAPGYSTGEAMAEIER
LVAQGPAGLGQEWTGLSLQERLSGSQAPILLGLSLLIVFLCLAALYESWSIPTSVLLVVP
LGVLGAVLAVTLRGMPNDVFFKVGLITIIGLSAKNAILIIEFAKSLYDEGHDLIDATLQA
ARLRLRPIVMTSLAFILGVVPLAIATGASSASQQAIGTGVIGGMITATLAVVFVPVFFVV
VMKLVRKRHKRI