Protein Info for Pf1N1B4_473 in Pseudomonas fluorescens FW300-N1B4

Annotation: DNA internalization-related competence protein ComEC/Rec2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 744 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 35 to 57 (23 residues), see Phobius details amino acids 230 to 250 (21 residues), see Phobius details amino acids 256 to 277 (22 residues), see Phobius details amino acids 301 to 320 (20 residues), see Phobius details amino acids 327 to 347 (21 residues), see Phobius details amino acids 359 to 378 (20 residues), see Phobius details amino acids 386 to 408 (23 residues), see Phobius details amino acids 415 to 434 (20 residues), see Phobius details amino acids 440 to 462 (23 residues), see Phobius details PF13567: DUF4131" amino acids 21 to 167 (147 residues), 105 bits, see alignment E=5.4e-34 TIGR00361: DNA internalization-related competence protein ComEC/Rec2" amino acids 112 to 714 (603 residues), 342.6 bits, see alignment E=3.8e-106 PF03772: Competence" amino acids 198 to 463 (266 residues), 192.5 bits, see alignment E=1.4e-60 TIGR00360: ComEC/Rec2-related protein" amino acids 219 to 397 (179 residues), 84.8 bits, see alignment E=8.4e-28 PF00753: Lactamase_B" amino acids 498 to 682 (185 residues), 64.5 bits, see alignment E=2e-21

Best Hits

KEGG orthology group: K02238, competence protein ComEC (inferred from 78% identity to pfo:Pfl01_4178)

Predicted SEED Role

"DNA internalization-related competence protein ComEC/Rec2"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MN28 at UniProt or InterPro

Protein Sequence (744 amino acids)

>Pf1N1B4_473 DNA internalization-related competence protein ComEC/Rec2 (Pseudomonas fluorescens FW300-N1B4)
MRTGMMALALGLLALRFLPVLPPAGLWLLLPVVGLMLLPFRTFPLAFLLFGFSWAGVNAQ
WVLNDRLAATLDGETRWLEGRVTGLPQQAEGVVRFELTESQSRRTRLPRHLRLAWYGGPP
VNSGEHWRLAVKLKRPAGLLNPHGFDYDAWLLARHIGATGTVKDGQRLMDAQWAWRDGIR
QRLQAVDAQGRTGALTALVLGDGAGLSREDWQVLQDTGTVHLLVISGQHIGLLAGVVYLL
IAGLARYGLWPKRLPWLPWACGLAFAAALGYGLLVGFEVPVRRACLMIGLVLLWRLRFRH
LGAWWPLLLALDGVLLLDPLASLQPGFWLSFAAVAVLIFTFGGRLGAWRWWQTWTRAQWL
IAIGLCPLLLVLGLPISLSGPLANLLAVPWVSLVVLPPALLGTMLLPVPYVGEGLLWLAG
GLIDLLFKGLALIAGQLPAWVPVAVPLWVWALGTLGAFLLLLPRGVPLRPLGWPMLLLLV
FSPRHTLPEGMAEVWQLDVGQGLAILVRTRHHTLLYDAGPRFGDIDLGERVVLPSLHKLG
VNGLDLMLISHADADHAGGARAIANGIPVKQILSGDHQALPDELHAESCESGRQWAWDDV
KFQLWQWPSAIDSNQKSCVLQIEANGERLLLTGDIDSAAERALLDGPLAVPTDWLQAPHH
GSRSSSSMALLSALQPNAVLISRGNGNSFGHPHPTVVARYQKRGVAIYDSAEQGAIRLQL
GRFKPPWTMLQERRFWRDPPPSSQ