Protein Info for Pf1N1B4_4726 in Pseudomonas fluorescens FW300-N1B4

Annotation: Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00871: glucose-6-phosphate dehydrogenase
  • amino acids 17 to 505 (489 residues), 564.4 bits, E=1e-173
• PF00479: G6PD_N
  • amino acids 21 to 205 (185 residues), 191.9 bits, E=1.7e-60
• PF02781: G6PD_C
  • amino acids 208 to 504 (297 residues), 385 bits, E=2e-119

Best Hits

KEGG orthology group: K00036, glucose-6-phosphate 1-dehydrogenase [EC: 1.1.1.49] (inferred from 92% identity to pfo:Pfl01_2587)

Predicted SEED Role

"Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49)" in subsystem Entner-Doudoroff Pathway or Pentose phosphate pathway (EC 1.1.1.49)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (16/17 steps found)
• aspartate superpathway (21/25 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• pentose phosphate pathway (8/8 steps found)
• heterolactic fermentation (15/18 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (15/18 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• Entner-Doudoroff pathway I (8/9 steps found)
• superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (7/8 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• pentose phosphate pathway (oxidative branch) I (3/3 steps found)
• cytosolic NADPH production (yeast) (4/5 steps found)
• L-lysine biosynthesis II (6/9 steps found)
• formaldehyde oxidation I (3/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Glutathione metabolism
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.49

Use Curated BLAST to search for 1.1.1.49

Sequence Analysis Tools

See A0A166Q7Q6 at UniProt or InterPro

Protein Sequence (507 amino acids)

>Pf1N1B4_4726 Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) (Pseudomonas fluorescens FW300-N1B4)
MTHVIRRKSKAEPAPPTTLFLFGAHGDLVKRLLMPALYNLSRDGLLGNGLRIIGVDHNAI
SDEAFAKKLEDFIRAEVAAKVGKGDQALDPALWAKLAKHISYVQGDFLDERTYEALAAKI
ADSGTGNAIFYLATAPRFFSEVVRRLGAAGLLQETPDAFRRVVIEKPFGSDLHTAEALNA
CLLKVMTEKQIYRIDHYLGKETVQNILVSRFSNSLFEAFWNNHYIDHVQITAAETVGVET
RGSFYEVTGALRDMVPNHLFQLLAMVAMEPPAAFGADAVRGEKAKVVGAIRPWSTEEARA
NSVRGQYTAGQINGEPLPGYRQEANVAPDSTTETYVALKVMIDNWRWVGVPFYLRTGKRM
SARDTEIVICFKPAPYAQFRDTEVDELQPTYLRIQIQPNEGMWFDLLAKRPGPALNMANI
ELGFAYKDFFEMQPSTGYETLIYDCLTGDQTLFQRADNIENGWRAVQPFLDAWQQDATVE
NYAAGEDGPKIADELLTRDGRVWHSVG