Protein Info for Pf1N1B4_4717 in Pseudomonas fluorescens FW300-N1B4

Annotation: Agmatinase (EC 3.5.3.11)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01230: agmatinase
  • amino acids 43 to 309 (267 residues), 247.5 bits, E=1e-77
• PF00491: Arginase
  • amino acids 44 to 309 (266 residues), 322 bits, E=1.9e-100

Best Hits

Swiss-Prot: 59% identical to SPEB_CHRVO: Agmatinase (speB) from Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)

KEGG orthology group: K01480, agmatinase [EC: 3.5.3.11] (inferred from 92% identity to pfo:Pfl01_2576)

MetaCyc: 56% identical to agmatinase (Escherichia coli K-12 substr. MG1655)
Agmatinase. [EC: 3.5.3.11]

Predicted SEED Role

"Agmatinase (EC 3.5.3.11)" in subsystem Arginine and Ornithine Degradation or Polyamine Metabolism (EC 3.5.3.11)

MetaCyc Pathways

• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
• L-arginine degradation III (arginine decarboxylase/agmatinase pathway) (2/2 steps found)
• putrescine biosynthesis I (2/2 steps found)
• superpathway of putrescine biosynthesis (3/4 steps found)
• superpathway of polyamine biosynthesis I (5/8 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.5.3.11

Use Curated BLAST to search for 3.5.3.11

Sequence Analysis Tools

See A0A166Q7G8 at UniProt or InterPro

Protein Sequence (319 amino acids)

>Pf1N1B4_4717 Agmatinase (EC 3.5.3.11) (Pseudomonas fluorescens FW300-N1B4)
MDVPAQNDQAMTRDSLYGTAAESTYAGITSFMRRRYSRDLRGVDVVVSGVPFDTATSNRP
GARFGPRGIRAASTGIAWERHWPWTFDPFDHLAVIDYGDCAFDYGTPQSVPESIEAHAEH
ILSGGCAMLTFGGDHFISYPLLKAHARQHGPLSLIHFDAHSDTWPDEEGKRVDHGTMFWH
AAKEGLVDPSRSVQIGLRTTNDDHQGFQVLDARQVHRQGCEAIVEAIRARVGDHPVYLTF
DIDCLDPAFAPGTGTPVCGGLSTVQALEILGGLRGINLVGMDVVEVAPAYDSADITSLAA
ATLAMEMLCLYVAKHKVDR