Protein Info for Pf1N1B4_4697 in Pseudomonas fluorescens FW300-N1B4
Annotation: Phenylacetate-coenzyme A ligase (EC 6.2.1.30)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 64% identity to pfo:Pfl01_2558)Predicted SEED Role
"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)
MetaCyc Pathways
- L-phenylalanine degradation IV (mammalian, via side chain) (5/9 steps found)
- penicillin G and penicillin V biosynthesis (1/4 steps found)
- phenylacetate degradation II (anaerobic) (1/4 steps found)
- superpathway of phenylethylamine degradation (6/11 steps found)
- phenylacetate degradation I (aerobic) (4/9 steps found)
- anaerobic aromatic compound degradation (Thauera aromatica) (4/27 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.30
Use Curated BLAST to search for 6.2.1.30
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A161Z829 at UniProt or InterPro
Protein Sequence (800 amino acids)
>Pf1N1B4_4697 Phenylacetate-coenzyme A ligase (EC 6.2.1.30) (Pseudomonas fluorescens FW300-N1B4) MYLINGQLRDDITPESALELLKKPLPRLLSSPIDSETVIESAVCFATKLQSRSQDLSLDD EQYQGLIDFCQRSNLSTKLERELGLQPRSLRRIDYRQPCFESWHPLGLVVHITPGNAPLL AFCAILESLLAGNINWLRPSASDQGLTAQLLAALVQCDSSGRLAEFVAVLPVDTSRIAQL CATADGVSAWGGVAALNAIRQQIPAGCRWIDWGHKISFAYLSPEAAEPAALDTLVDEVCR LDQQACSSPQWVLVDSNDPAVLHALGERLAEAFKRRAQQWPALMPTDQEASEITTRTAMA QLDQCFTDQTGQVWTGSGWRVIWEHHQTLAPSPLFRTVLLKPVPQHLLAESLLSWRNVLQ SCALIASPAGTPELVRTLVNAGVTRIAPCETIHDGYAGEPHDGVYALSRLSRRLSVSLAA DVSIGRSTLDPVPSAPDTARGPIMDKDTFTTQPISPTAQLYFRSGGSSGTPALAGFSYRD FQRQMRAAADGMFAVGLDPSQDRVMNLFYGGNLYGGFFSFSSILEQMGVAHLPMGAPQDD DFSEIARLIVEQRVTVLIGMPSTLHRLFFKEQARLRDYAGIGKVLLGGEHPGEASRSLME SCGVSTIRSALYGSVDAGPLGHACRATADGVFHLMCETQYLEIVQLEQDVPVQANEIGRL LFTSRARQVQRVHRYDIGDTGRWLPGACPCGLETPRFELLQRHGKLVRIGTEFISPSALE HSVGVPIQVVLDYTASGVERMQVRTDADITWVREKLLTHEALANAIAAALLVVEVIACAE HEFIRNKHSGKVPLIVDRRI