Protein Info for Pf1N1B4_4677 in Pseudomonas fluorescens FW300-N1B4

Annotation: Trehalose synthase (EC 5.4.99.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1113 TIGR02456: trehalose synthase" amino acids 16 to 563 (548 residues), 933.1 bits, see alignment E=4.4e-285 PF00128: Alpha-amylase" amino acids 39 to 399 (361 residues), 250.8 bits, see alignment E=4.2e-78 PF16657: Malt_amylase_C" amino acids 478 to 560 (83 residues), 88.7 bits, see alignment 3.2e-29 TIGR02457: putative maltokinase" amino acids 580 to 1100 (521 residues), 621.7 bits, see alignment E=1.1e-190 PF18085: Mak_N_cap" amino acids 612 to 690 (79 residues), 30.4 bits, see alignment 1e-10

Best Hits

KEGG orthology group: K05343, maltose alpha-D-glucosyltransferase [EC: 5.4.99.16] (inferred from 71% identity to avn:Avin_28000)

Predicted SEED Role

"Trehalose synthase (EC 5.4.99.16)" in subsystem Trehalose Biosynthesis (EC 5.4.99.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z812 at UniProt or InterPro

Protein Sequence (1113 amino acids)

>Pf1N1B4_4677 Trehalose synthase (EC 5.4.99.16) (Pseudomonas fluorescens FW300-N1B4)
MAKKPRSATFIKDPLWYKDAVIYQVHVKSFFDSNNDGIGDFPGLIAKLDYISDLGVNTIW
LLPFYPSPRRDDGYDIAEYRGVHPDYGTMADAKRFIAEAHKRGLRVITELVINHTSDQHA
WFQRARKAKPGSAARDFYVWSDNDQKYDGTRIIFLDTEKSNWTWDPVAGQYFWHRFYSHQ
PDLNFDNPQVMKAVLSVMRYWLDMGIDGLRLDAIPYLIERDGTNNENLPETHDVLKQIRA
EIDANYPDRMLLAEANQWPEDTQLYFGNTDASGQKGDECHMAFHFPLMPRMYMALAQEDR
FPITDILRQTPEIPANCQWAIFLRNHDELTLEMVTDRERDYLWNYYAADRRARINLGIRR
RLAPLMERDRRRVELLNSLLLSMPGTPTLYYGDEIGMGDNIYLGDRDGVRTPMQWSIDRN
GGFSRADPASLVLPPIMDPLYGYLSVNVETQAGDPHSLLNWNRRLLAVRKQSKAFGRGTL
KMLSPSNRRILAYTREFTGPDGKHEIILCVANVSRSAQAAELDLSAYVGMVPVEMLGGNA
FPPIGQLNFLLTLAPYGFYWFGLAAENQMPSWHVEPAQSLPDFTTLVLKKRMEELLEAPS
RHTLEQGILPSWLQNRRWYAGKDGVIDSVNLAYGVRFGDPQHPVLLGEIDVTSGGQTSRY
QLPFGFISEEQVGAALPQQLALARVRRGRQVGLITDAFSLDSFVHAVLQSMQAGMVLPSD
GGEIRFEPADELTRLGLTAESEVRYLSAEQSNSSVVIGSSLVLKLIRKVAPGVHPELEMS
AYLTHAGFQNISPLLGAVIRRNAAGEDTLLMIAQGYLSNQGDAWEWTQNNLERALRDELA
DAVSEQEQHYNALGELKDFAGMLGQRLGEMHRVLAAPSANPDFAPQITTQKDALASAKDV
AAQLEHALKLLKQHQTELNAADQALVSRLLDNKKAILSHVQELGKKAAGGLRIRVHGDLH
LGQVLVIKGDAYLIDFEGEPARPLHERRGKHSPYKDVSGVLRSFDYAAAMTINVHNVDNT
VEAQAARQRVADRYLKEARQAFVDAYRLAAASLAHAWQDPEGEDAALALFGLEKAAYEVA
YEAENRPTWLPVPLHGLYGLLSGLKPFSDLGGE