Protein Info for Pf1N1B4_4676 in Pseudomonas fluorescens FW300-N1B4

Annotation: Alpha-amylase (EC 3.2.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 665 PF11896: GlgE_dom_N_S" amino acids 24 to 206 (183 residues), 175.4 bits, see alignment E=2.3e-55 PF00128: Alpha-amylase" amino acids 230 to 335 (106 residues), 32.3 bits, see alignment E=1.3e-11 PF21702: GLGE_C" amino acids 578 to 661 (84 residues), 64.3 bits, see alignment E=1.5e-21

Best Hits

Swiss-Prot: 66% identical to GLGE_PSEAE: Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase (glgE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 88% identity to pfo:Pfl01_2534)

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162B011 at UniProt or InterPro

Protein Sequence (665 amino acids)

>Pf1N1B4_4676 Alpha-amylase (EC 3.2.1.1) (Pseudomonas fluorescens FW300-N1B4)
MTAEKPTELSYNPHIPLSQALLLPRIVIENTMPTLDGGQFAVKAVIDQDVVVTSKVFADG
HDKLAVRIRWRAEGDETWQSEVMAELGNNGWQGQFRVQKQGRYVFCLEAWIDQFASFCYE
LKKKHDAGVSVSLELQEGRTHVQQAAERSEGPLSEQLAALHHELSGLLETEQVALFLHQR
SAGLMAQADHRPFLSLSPEYPVDVERELAQFASWYELFPRSITDDPARHGTFNDVHSRLA
MIQDMGFDVLYFTPIHPIGRSYRKGPNNSLIAGPDDPGSPYAIGSEEGGHEAIHSELGTR
EDFRRLVAAAAEHGLEVALDFAIQCSQDHPWLKQHPGWFNWRPDGTIKYAENPPKKYQDI
VNVDFYAVDAIPGLWIELRDIVVGWVKEGVKLFRVDNPHTKPLPFWQWMIADVRALYPEV
IFLAEAFTTPAMMARLGKVGYSQSYTYFTWRNTKTELATYFTELNESPWRECYRPNFFVN
TPDINPTFLHESGRAGFLIRAALATMGSGLWGMYSGFELCEAAPVPGREEYLNSEKYEIR
PRDFNAPGNIIAEIAQLNRIRRQNPALHTHLGLKVYNAWNDNILYFGKRSVDGSNFILVA
VSLDPHHAQEANFELPLWEMGLPDDAVTQGEDLMNGHRWTWHGKYQFMRIEPWHLPFGIW
RITSS