Protein Info for Pf1N1B4_4669 in Pseudomonas fluorescens FW300-N1B4

Annotation: Heavy metal RND efflux outer membrane protein, CzcC family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 307 transmembrane" amino acids 253 to 268 (16 residues), see Phobius details PF02321: OEP" amino acids 2 to 87 (86 residues), 31.6 bits, see alignment E=7.2e-12 amino acids 111 to 294 (184 residues), 127.6 bits, see alignment E=2.7e-41 TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 2 to 296 (295 residues), 297.4 bits, see alignment E=9.7e-93

Best Hits

Predicted SEED Role

"Heavy metal RND efflux outer membrane protein, CzcC family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (307 amino acids)

>Pf1N1B4_4669 Heavy metal RND efflux outer membrane protein, CzcC family (Pseudomonas fluorescens FW300-N1B4)
MRLSQQSELVQNYLQLRVIDEQKRLLEATVEAYQRSLTMTENQYRAGVSGKDAVAQATTQ
LKSTQAEMVDLIWQRAQFENAIAVLIGLPPAEFSLAETQDIPALPQVPLSLPSQLLERRP
DIASAERSVMAANANIGVAKAAYYPDLTLSLNGGYSSSTSNNWISVPNRFWSVGPQLAMT
LFDGGQRSAEVDRSEAAYDETVAKYRQTVLDGFREVENYLVQLKVLEDEAKVRQEALEAA
RESLRLTRNQYRAGLIAYLDVVVVQATALSNERNVLSLLQSRLIASVQLIAALGGGWDGQ
LQVSDKQ