Protein Info for Pf1N1B4_4643 in Pseudomonas fluorescens FW300-N1B4
Annotation: NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 83% identical to DHE2_PSEAE: NAD-specific glutamate dehydrogenase (gdhB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Predicted SEED Role
"NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)
MetaCyc Pathways
- L-alanine degradation II (to D-lactate) (3/3 steps found)
- L-glutamate degradation I (1/1 steps found)
- ethene biosynthesis IV (engineered) (2/3 steps found)
- L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
- L-glutamate degradation XI (reductive Stickland reaction) (3/7 steps found)
- 4-aminobutanoate degradation V (2/7 steps found)
- methylaspartate cycle (9/19 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.4.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166Q5Q0 at UniProt or InterPro
Protein Sequence (1635 amino acids)
>Pf1N1B4_4643 NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form (Pseudomonas fluorescens FW300-N1B4) MAFFTAASKADFQHQLQAALAQHISEQALPQVALFAEQFFGIISLDELTQRRLSDLAGCT LSAWRLLERFDHAHPQVRVYNPDYERHGWQSTHTAVEVLHHDLPFLVDSVRTELNRRGYS IHTLQTTVLSVRRGSKGELLEILPKGTQGEGILQESLMYLEIDRCANTAELNVLTKELEQ VLGEVRVAVADFEPMKAKVQELIEGIDNSQFIINADEKSEIKSFLEWLVGNHFTFLGYEE FVVGEDQNGGHIEYDQNSFLGLTKLLRAGLTVDDLRIEDYAVNYLREPTPLSFAKAAHPS RVHRPAYPDYVSIRQIDANGKVVKECRFMGLYTSSVYGESVRVIPYIRRKVEIIEQRSGF QAKAHLGKELAQVLEVLPRDDLFQTPVDELFTTVMSIVQIQERNKIRVFLRKDPYGRFCY CLAYVPRDIYSTEVRQKIQQVLMDRLKASDCEFWTFFSESVLARVQLILRVDPKNRLDID PVLLEKEVVQACRSWQDDYASLVVESFGEAQGTNVLADFPKGFPAGYRERFAAHSAVVDM QHLLSLSEKNPLVMSFYQPLAQVSGQRMLHCKLYHADTPLALSDVLPILENLGLRVLGEF PYRLRHNSGREFWIHDFAFTAAEGLELDIQQLNDTLQDAFVHIVRGDAENDAFNRLVLTA GLPWRDVALLRAYARYMKQIRLGFDLGYIASTLNNHTDIARELTRLFKTRFYLARKLASD DLEDKQQRLEHAILAALDDVQVLNEDRILRRYLDLIKATLRTNFYQTDTHGQNKSYFSFK FNPHQIPELPKPVPKFEIFVYSPRVEGVHLRFGNVARGGLRWSDREEDFRTEVLGLVKAQ QVKNSVIVPVGAKGGFLPRRLPLGGSRDEIAAEGIACYRIFISGLLDITDNLKDGALVPP ANVVRHDDDDPYLVVAADKGTATFSDIANGIAIDYGFWLGDAFASGGSAGYDHKKMGITA KGAWVGVQRHFRERGINVQEDSITVVGVGDMAGDVFGNGLLMSDKLQLVAAFNHLHIFID PNPEPANSFAERQRLFDLPRSAWSDYDTSIMSEGGGIFSRSAKSIAISPQMKERFDIQAD KLTPTELLNALLKAPVDLLWNGGIGTYVKASTESHADVGDKANDALRVNGNELRCKVVGE GGNLGMTQLGRVEFGLNGGGSNTDFIDNAGGVDCSDHEVNIKILLNEVVHAGDMTDKQRN QLLASMTDEVGNLVLGNNYKQTQALSLAARRAFVRIAEYKRLMNDLEGRGKLDRAIEFLP AEEAINERLAEGHGLTRAELSVLISYSKIDLKEALLNSLVPDDDYLTRDMETAFPPTLVS KFSAAMRRHRLKREIVSTQIANDLVNHMGITFVQRLKESTGMSPANVAGAYVIVRDIFHL PHWFRQIEALDYQVSADVQLELMDELMRLGRRATRWFLRSRRNEQNAARDVAHFGPHLAA LGLKLDELLEGPTREGWQTRYQAYVAAGVPELLARMVAGTTHLYTLLPIIEASDVTGQNA ADVAKAYFAVGSALDITWYLQQISALPVENNWQALAREAFRDDVDWQQRAITISVLQQGD GTQDVETRLALWMEEHEGMIERWRAMLVEIRAASGTDYAMYAVANRELLDLALSGQAVVP AAATAAVSTELEPAA