Protein Info for Pf1N1B4_4614 in Pseudomonas fluorescens FW300-N1B4

Annotation: Membrane alanine aminopeptidase N (EC 3.4.11.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 885 TIGR02414: aminopeptidase N" amino acids 13 to 884 (872 residues), 1330.1 bits, see alignment E=0 PF17900: Peptidase_M1_N" amino acids 56 to 192 (137 residues), 62.4 bits, see alignment E=1.3e-20 PF01433: Peptidase_M1" amino acids 232 to 445 (214 residues), 186 bits, see alignment E=1.6e-58 PF11940: DUF3458" amino acids 450 to 554 (105 residues), 106 bits, see alignment E=2.2e-34 PF17432: DUF3458_C" amino acids 559 to 885 (327 residues), 406.5 bits, see alignment E=1.9e-125

Best Hits

KEGG orthology group: K01256, aminopeptidase N [EC: 3.4.11.2] (inferred from 95% identity to pfo:Pfl01_2200)

Predicted SEED Role

"Membrane alanine aminopeptidase N (EC 3.4.11.2)" (EC 3.4.11.2)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166Q566 at UniProt or InterPro

Protein Sequence (885 amino acids)

>Pf1N1B4_4614 Membrane alanine aminopeptidase N (EC 3.4.11.2) (Pseudomonas fluorescens FW300-N1B4)
MRTEQPKMIYLKDYQAPEYLIDETHLTFELFEDHSLVHAQLVMRRNPERGPGLPPLVLDG
QQLDLLSVTLADRELSAADYQLTENHLTLHPASTSFTVDTSVRIHPETNTALEGLYKSGT
MFCTQCEAEGFRKITYYLDRPDVMSTFTTTVVAEQHSYPVLLSNGNPIASGPGEDGRHWA
TWEDPFKKPAYLFALVAGDLWCVEDTFTTMTERSVALRIYVEPENIDKCQHAMNSLKKSM
RWDEEVYGREYDLDIFMIVAVNDFNMGAMENKGLNIFNSSAVLARAETATDAAHQRVEAI
VAHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDSEFSADMNSATVKRIQDVAYLRTHQFA
EDAGPMAHAVRPDSFIEISNFYTLTVYEKGSEVVGMIHTLLGADGFRKGSDLYFERHDGQ
AVTCDDFIKAMEDANGVDLTQFKRWYSQAGTPRLAVSESYDAAAKTYSLTFRQSCPETPD
KVEKLPFVIPVELGLLDSKGDEIALRLAGETSAQGTSRVISVTEAEQTFTFIDIAQKPLP
SLLRGFSAPVKLSFPYDRDQLMFLMQHDSDGFNRWDAGQQLSVQVLQELIEQQQKGEALV
LDQRLVSALRTVLSDETLDQAMVAEMLSLPGEAYLTEISEVADVDAIHTAREFARKQLAE
GLFEALWLRYQANRDLSKKTPYVAEAEHFARRALQNIALSYLMLSGKPEVLAATLEQFDT
SDNMTERLTALAVLVNSPFEEQKATALATFAEHFKDNPLVMDQWFSVQAGSTLPGGLERV
KALMQHPAFTIKNPNKVRALIGAFAGQNLINFHAADGSGYRFLADLVIELNGFNPQIASR
QLAPLTRWRKYDDVRQALMKGELERIRASGQLSSDVFEVVSKSLA