Protein Info for Pf1N1B4_457 in Pseudomonas fluorescens FW300-N1B4

Annotation: Periplasmic serine proteases (ClpP class)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 transmembrane" amino acids 52 to 73 (22 residues), see Phobius details TIGR00706: signal peptide peptidase SppA, 36K type" amino acids 87 to 290 (204 residues), 158 bits, see alignment E=1.3e-50 PF01343: Peptidase_S49" amino acids 153 to 293 (141 residues), 114.8 bits, see alignment E=1.9e-37

Best Hits

KEGG orthology group: K04773, protease IV [EC: 3.4.21.-] (inferred from 89% identity to pfo:Pfl01_4163)

Predicted SEED Role

"Periplasmic serine proteases (ClpP class)"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QH70 at UniProt or InterPro

Protein Sequence (338 amino acids)

>Pf1N1B4_457 Periplasmic serine proteases (ClpP class) (Pseudomonas fluorescens FW300-N1B4)
MGFFAGVYGMTDEWKAPAKASAETGDEKSWKLLEKTLLAGVQEQRRSRRWGIFFKSLTFL
YLFGLLVLLSPMISMEKGAALGSGYTALIDITGMIADKEPASADNIVGSLRAAFEDKKVK
GVVLRINSPGGSPVQSGYVYDEIRRLRGLHPDTKVYAVISDLGASGAYYIASAADQIYAD
KASLVGSIGVTAAGYGFVGTMEKLGVERRTYTSGEHKSFLDPFQPQKPAEIQFWQSVLDT
THQQFINSVKKGRGDRLKDKEHPELFSGLIWSGEQALSLGLIDGLGNASSVARDVIGEKE
LVDFTVEESPFDRFSKKLGASVAEHLAMWMGFQGPSLR