Protein Info for Pf1N1B4_4556 in Pseudomonas fluorescens FW300-N1B4

Annotation: D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 235 PF13561: adh_short_C2" amino acids 8 to 231 (224 residues), 170.6 bits, see alignment E=6.5e-54 PF00106: adh_short" amino acids 9 to 171 (163 residues), 133.3 bits, see alignment E=1.2e-42 PF08659: KR" amino acids 34 to 133 (100 residues), 27 bits, see alignment E=6.1e-10

Best Hits

KEGG orthology group: None (inferred from 86% identity to ppu:PP_2784)

Predicted SEED Role

"D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30)" in subsystem Polyhydroxybutyrate metabolism (EC 1.1.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.30

Use Curated BLAST to search for 1.1.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166Q419 at UniProt or InterPro

Protein Sequence (235 amino acids)

>Pf1N1B4_4556 D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) (Pseudomonas fluorescens FW300-N1B4)
VAENFATDAGNRLILLDLDLPQLQDWVAGQVFAASIETYRADIADLASLQVLFKTLATRL
GYVDVLVNSAGVCDENEPEDLDNWHKVISVNLNGTFYVTSLCLPLMADRGRIINMSSILG
RAGKVRNTAYCASKHGIIGLTKALAMDLAPRQITVNAILPAWIDTPMLQGELAAQARIAG
ISQEQILRNAKKKLPLRRFIQGDEVAAMVRYLASPEAGGVTAQSLMIDGGVGLGM