Protein Info for Pf1N1B4_4523 in Pseudomonas fluorescens FW300-N1B4

Annotation: RNA polymerase sigma-70 factor, ECF subfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 8 to 160 (153 residues), 64.5 bits, see alignment E=4.6e-22 PF04542: Sigma70_r2" amino acids 11 to 73 (63 residues), 49.7 bits, see alignment E=2.6e-17 PF08281: Sigma70_r4_2" amino acids 110 to 159 (50 residues), 46.6 bits, see alignment 2.1e-16

Best Hits

KEGG orthology group: K03088, RNA polymerase sigma-70 factor, ECF subfamily (inferred from 82% identity to pfo:Pfl01_3436)

Predicted SEED Role

"RNA polymerase sigma-70 factor, ECF subfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AZQ2 at UniProt or InterPro

Protein Sequence (297 amino acids)

>Pf1N1B4_4523 RNA polymerase sigma-70 factor, ECF subfamily (Pseudomonas fluorescens FW300-N1B4)
MNSAENAAAVFEQRRPFLLGLAYRILGSRTEAEDVVQELFIKWLEADRASIATPAAWLTT
ACTRHCIDLLRSAHTSRVDYVGTWLPEPIHTTHQHSPEHMHELASSLSTAFLLLLERLTP
KERAAYLLYEIFDMDYAQVAEAIGVQEAACRKLVSRAKAGLGSGQIRYQPEPARQDQLLS
AFHSAIASGSTASLTALLAEDVELCADGGGKASAIRETLSGITSVLDFIGQSLHVYWHTC
EWLPSEINGAQGVIIKADGVITASMTFGFDEAGRVSRIFIMRNPDKLAGLDASLQVQ