Protein Info for Pf1N1B4_452 in Pseudomonas fluorescens FW300-N1B4

Annotation: Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 62% identical to FABD_SALTY: Malonyl CoA-acyl carrier protein transacylase (fabD) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00645, [acyl-carrier-protein] S-malonyltransferase [EC: 2.3.1.39] (inferred from 96% identity to pba:PSEBR_a4052)

MetaCyc: 61% identical to [acyl-carrier-protein] S-malonyltransferase (Escherichia coli K-12 substr. MG1655)
[Acyl-carrier-protein] S-malonyltransferase. [EC: 2.3.1.39]

Predicted SEED Role

"Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 2.3.1.39)

MetaCyc Pathways

superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
superpathway of fatty acid biosynthesis I (E. coli) (15/16 steps found)
fatty acid biosynthesis initiation (type II) (3/3 steps found)
superpathway of fatty acid biosynthesis initiation (4/5 steps found)
fatty acid biosynthesis initiation (mitochondria) (2/4 steps found)
fatty acid biosynthesis initiation (plant mitochondria) (1/4 steps found)
mycobactin biosynthesis (2/11 steps found)
pederin biosynthesis (2/14 steps found)
bryostatin biosynthesis (2/19 steps found)
mupirocin biosynthesis (2/26 steps found)
corallopyronin A biosynthesis (2/30 steps found)

KEGG Metabolic Maps

Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.39

Use Curated BLAST to search for 2.3.1.39

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z3Z5 at UniProt or InterPro

Protein Sequence (294 amino acids)

>Pf1N1B4_452 Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39) (Pseudomonas fluorescens FW300-N1B4)
MLAELGAQHPVVLETFKEASDALGYDLWALTQQGPEEQLNQTDKTQPAILTASIALWRLW
LAEGGARPAYVAGHSLGEYSALVAAGSLSLGDAVKLVERRGQLMQEAVPAGQGGMAAILG
LEDADVLAACAEAAQGEVVSAVNFNSPGQVVIAGAKAAVERAIEGCKARGAKRAMPLPVS
VPSHCELMRPAAERFAESIAAIDWQAPQIPVVQNVSATVPADLETLKRDLLEQLYKPVRW
VESVQTLAAKGATQLVECGPGKVLAGLNKRCAEGVSTSNLNTPDAFAAARAALA