Protein Info for Pf1N1B4_4461 in Pseudomonas fluorescens FW300-N1B4

Annotation: Glutathione-regulated potassium-efflux system protein KefB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 602 transmembrane" amino acids 6 to 25 (20 residues), see Phobius details amino acids 32 to 51 (20 residues), see Phobius details amino acids 57 to 76 (20 residues), see Phobius details amino acids 88 to 111 (24 residues), see Phobius details amino acids 117 to 138 (22 residues), see Phobius details amino acids 150 to 173 (24 residues), see Phobius details amino acids 187 to 206 (20 residues), see Phobius details amino acids 226 to 256 (31 residues), see Phobius details amino acids 272 to 293 (22 residues), see Phobius details amino acids 297 to 320 (24 residues), see Phobius details amino acids 329 to 349 (21 residues), see Phobius details amino acids 361 to 383 (23 residues), see Phobius details PF00999: Na_H_Exchanger" amino acids 13 to 378 (366 residues), 222.7 bits, see alignment E=7.1e-70 TIGR00932: transporter, monovalent cation:proton antiporter-2 (CPA2) family" amino acids 15 to 286 (272 residues), 289.6 bits, see alignment E=1.4e-90 PF02254: TrkA_N" amino acids 409 to 522 (114 residues), 90.9 bits, see alignment E=7.4e-30

Best Hits

KEGG orthology group: K11747, glutathione-regulated potassium-efflux system protein KefB (inferred from 93% identity to pfo:Pfl01_3513)

Predicted SEED Role

"Glutathione-regulated potassium-efflux system protein KefB" in subsystem Glutathione-regulated potassium-efflux system and associated functions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161ZCJ1 at UniProt or InterPro

Protein Sequence (602 amino acids)

>Pf1N1B4_4461 Glutathione-regulated potassium-efflux system protein KefB (Pseudomonas fluorescens FW300-N1B4)
MPHEGNLLQAAVVFLFAAVLTVPLAKRLQLGAVLGYLFAGVIIGPSVLGLIGNPQSVSHI
SELGVVLLLFIIGLELSPRRLWVMRKSVFGVGLAQVLLTGSVIGVLALFVFGQSLNSAIV
LGLGLALSSTAFGLQSLAERKELTSPHGRLAFAILLFQDIAAIPLIAMVPLLAGGDHTTS
AAEDLNHGLRVLGSIAVVVIGGRYLLRPVFRVVAKTGLPEVSTATALLVVIGTAWLMDLV
GVSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMGANLSLLLSAPVVVL
GLTLLLIAIKLPLLFVVGRLAGGLGKVSAIRLGIVLAAGGEFAFVVFKIGRDQGLFEPRL
YDLLVLTITLSMAVTPLLLLLCARLVSPKVQPVVVPEKFREIDADTPRVVIAGMGRMGQI
VARILRAQNIKFIALDTSVETIELSRSFGGVPVFYGDPMRPEILSAAKVEQAEFFVIATD
DPDTNIKTAEVVHKLYPHIKIIARARNRQHVHRLVDVGAQAVRETYYSSLEMTRRTLVGL
GLTQAQADARIKRFTHHDEQVLEAQHAVYDDAAKVMQTAQEARAELAKLFESDQLEEEAG
KV