Protein Info for Pf1N1B4_4430 in Pseudomonas fluorescens FW300-N1B4

Annotation: Cystathionine beta-lyase (EC 4.4.1.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 919 PF00581: Rhodanese" amino acids 6 to 101 (96 residues), 45.4 bits, see alignment E=9.6e-16 amino acids 131 to 223 (93 residues), 44.7 bits, see alignment E=1.6e-15 amino acids 280 to 355 (76 residues), 34.9 bits, see alignment E=1.8e-12 amino acids 375 to 463 (89 residues), 30 bits, see alignment E=6.1e-11 PF01053: Cys_Met_Meta_PP" amino acids 537 to 914 (378 residues), 338 bits, see alignment E=5.4e-105 TIGR01324: cystathionine beta-lyase" amino acids 537 to 916 (380 residues), 417.1 bits, see alignment E=2.7e-129

Best Hits

KEGG orthology group: K01760, cystathionine beta-lyase [EC: 4.4.1.8] (inferred from 88% identity to pfs:PFLU2239)

Predicted SEED Role

"Cystathionine beta-lyase (EC 4.4.1.8)" in subsystem Methionine Biosynthesis (EC 4.4.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.4.1.8

Use Curated BLAST to search for 4.4.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161ZCG0 at UniProt or InterPro

Protein Sequence (919 amino acids)

>Pf1N1B4_4430 Cystathionine beta-lyase (EC 4.4.1.8) (Pseudomonas fluorescens FW300-N1B4)
MNRTVTPGQLQQWLFDGQEIALFDVREHGQYGEAHLFHGVNLPYSRLELEVRRLAPNPQV
RLVIYDQNGGDVVARAAQRLQALGYGRVHILDGGADGWQAAGLQLFAGVHVPSKAFGELV
EEVSHTPHVTARQLAEWQARGESLVVLDGRPFDEYRKMTIPGSVCCPNGELGYRVHDLVP
DDSTPIVVNCAGRTRSIIGAQTLINLGLKNPVYALENGTQGWDLEDLQLEHGSTRRYADE
VSSKTLPLQRLAAAQLAERAGVKTVATAQVEQWAGEAERSLFLCDVRTAEEFAAGSLPGA
QHTPGGQLIQSTDLYIGVRQARVVLIDSDGVRAPIIASWLRQLGHEAYVLADGVSSGLAL
PAVETPAHEALPVITAQALSDALKDNAVALIDLRPSMTWRKGHIAGSRWSIRSLLATEVM
GEQRPLVLLADDPLLAAFATLELPNAQRTQTRLLDGGVEAWRAAGFALQEDANTPPDHQC
IDFLFFTHDRHSGNKDAARQYLAWEIGLLAQMSADEIASLKPLRAQSQATRDARIRTRLV
HSARTEKGSGGRAVNVPVTRLSTVLFDNLAQMRDARARRDSERVLSYGARGNPTAFALED
LVTELEGGYRTRLYGTGLAAVAQTFLAYLRPGDHVLITDAVYAPVRRLARELLEPFGIQV
SYFAPDGSGLQAQLQANTKMVYTEVPGSLLYELCDLPAIAALCKPRGILLAVDNTWGSGY
LYRPLTLGADISIMALTKYLCGHSDVVMGSVCTRQEVWQPLSSMSDTFGIAVSPDDAYLV
LRGARTLAPRLDVHERQALEIAQWLQAQPQVKRVFHPALPDHPHHALWRRDFNGSNGLLS
FELREADATYVERFIDALQVFGLGASWGGYESLVTVADTKDRNSAADRALNPVLRLHIGL
EDVDALIEDLQRGFAAAVD