Protein Info for Pf1N1B4_4351 in Pseudomonas fluorescens FW300-N1B4

Annotation: Putrescine transport system permease protein PotH (TC 3.A.1.11.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 304 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details amino acids 85 to 109 (25 residues), see Phobius details amino acids 121 to 142 (22 residues), see Phobius details amino acids 173 to 192 (20 residues), see Phobius details amino acids 227 to 246 (20 residues), see Phobius details amino acids 271 to 292 (22 residues), see Phobius details PF00528: BPD_transp_1" amino acids 102 to 290 (189 residues), 37 bits, see alignment E=1.5e-13

Best Hits

Swiss-Prot: 60% identical to POTH_ECOLI: Putrescine transport system permease protein PotH (potH) from Escherichia coli (strain K12)

KEGG orthology group: K11075, putrescine transport system permease protein (inferred from 94% identity to pfo:Pfl01_2119)

MetaCyc: 60% identical to putrescine ABC transporter membrane subunit PotH (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine transport system permease protein PotH (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166PZF7 at UniProt or InterPro

Protein Sequence (304 amino acids)

>Pf1N1B4_4351 Putrescine transport system permease protein PotH (TC 3.A.1.11.2) (Pseudomonas fluorescens FW300-N1B4)
MRTFNQQFLRLVPSGRKLVIGIPFLWLCLFFLLPFFLVMKISFSEAALAIPPYSEIYTFA
EQKFQLLLNIGNYSLLTEDELYISAYFGSLKVAFLSTLMCLVIGFPMAYAITKANKETQN
VLLLLIMMPTWTAILIRVYAWMGILSNNGLLNAFLMWTGLTSHPIEILNTNTAVYIGVVY
AYLPFMVLPLYANLVKHDHSLLEAASDLGSSNFNNFWKITVPLAKNGIIAGCMLVFIPVV
GEFVIPELLGGPETLMIGRVLWQEFFNNRDWPVASALAVVMLAILIVPILLFNRSQAKEM
EGRG