Protein Info for Pf1N1B4_4327 in Pseudomonas fluorescens FW300-N1B4

Annotation: Spermidine/putrescine-binding periplasmic protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details PF01547: SBP_bac_1" amino acids 47 to 310 (264 residues), 44.6 bits, see alignment E=3.9e-15 PF13416: SBP_bac_8" amino acids 55 to 331 (277 residues), 109.3 bits, see alignment E=6.2e-35 PF13343: SBP_bac_6" amino acids 93 to 329 (237 residues), 59.1 bits, see alignment E=1e-19

Best Hits

Swiss-Prot: 42% identical to POTF_ECOLI: Putrescine-binding periplasmic protein (potF) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 88% identity to pfo:Pfl01_2372)

MetaCyc: 42% identical to putrescine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Spermidine/putrescine-binding periplasmic protein"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161ZC73 at UniProt or InterPro

Protein Sequence (377 amino acids)

>Pf1N1B4_4327 Spermidine/putrescine-binding periplasmic protein (Pseudomonas fluorescens FW300-N1B4)
MHAGTLKGFDMRMSNKLLIGALGLALGGALPSLHAQEKTLRLYNWADYFAEDTLTRFTAE
TGIKVIYDVMDGSETLEAKMLAGGSGYDLIFPGDTVAERLMRAGSLLPLDRSKLTALDDI
EPGLQKLRTHYVHSNQATVPYTWGTIGLTYNAEQIKQRMPDAPVNSLDMLFKPELAARFA
DCGISMIDSPDEVLAVALNYLGREPRSAKPEDLAAASELLLKLRPYIRKFQSQPVTDLVN
GNLCLSLGYSGDMTQAQRTADTAGKKTTFQYRIPREGTTVWMDTMAIPVDARHPEYAYAF
INFVMRPQNMAAISNFTGYPTSNAKARPSVDATMRNNPDIYLDEATYERLIPGKDIPQPD
MRARMRTWTKFKTATTK