Protein Info for Pf1N1B4_4309 in Pseudomonas fluorescens FW300-N1B4

Annotation: Translation elongation factor Tu

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 305 PF00009: GTP_EFTU" amino acids 8 to 201 (194 residues), 196.5 bits, see alignment E=5e-62 TIGR00231: small GTP-binding protein domain" amino acids 10 to 142 (133 residues), 51.7 bits, see alignment E=4.3e-18 PF03143: GTP_EFTU_D3" amino acids 214 to 303 (90 residues), 123.1 bits, see alignment E=9.3e-40

Best Hits

Predicted SEED Role

"Translation elongation factor Tu" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AZ70 at UniProt or InterPro

Protein Sequence (305 amino acids)

>Pf1N1B4_4309 Translation elongation factor Tu (Pseudomonas fluorescens FW300-N1B4)
VNTPFEGPHVNVGTIGHIGHGKTTLTAALTRVCSEVFGSAKVDFDKIDSAPEENVDGITI
NIAHVEYDSAVRHYAHVDCPGHADYVKSMITGAAQMDGAILVCSAADGPMPQTREHIQLS
RQVGVPYIVVFLNKADMVDDAELLELLEMEVRYLLSTYGFPGDDTPIIIGSALMALNGQD
DNKMGTTAVKKLVETLDSYIPDPVRVIDKPGELHIKFTAEVYVLSKEEGGRHTPFFNGYH
PRFYFRTTDVTGLCELPEVEMVMPGDNVQMTVTLIQPIAMENGLRFAIREDDRTVGAGVV
VKIIE