Protein Info for Pf1N1B4_4281 in Pseudomonas fluorescens FW300-N1B4

Annotation: Glyceraldehyde-3-phosphate ketol-isomerase (EC 5.3.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01261: AP_endonuc_2
  • amino acids 19 to 276 (258 residues), 172 bits, E=9.7e-55

Best Hits

Swiss-Prot: 62% identical to IOLH_BACSU: Protein IolH (iolH) from Bacillus subtilis (strain 168)

KEGG orthology group: K06605, myo-inositol catabolism protein IolH (inferred from 87% identity to psb:Psyr_3267)

Predicted SEED Role

"Glyceraldehyde-3-phosphate ketol-isomerase (EC 5.3.1.1)" in subsystem Inositol catabolism (EC 5.3.1.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• gluconeogenesis I (11/13 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
• homolactic fermentation (10/12 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• glycolysis III (from glucose) (9/11 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• glycolysis IV (8/10 steps found)
• Calvin-Benson-Bassham cycle (10/13 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• sucrose degradation V (sucrose α-glucosidase) (3/5 steps found)
• gluconeogenesis III (8/12 steps found)
• glycolysis VI (from fructose) (7/11 steps found)
• oxygenic photosynthesis (11/17 steps found)
• glycerol degradation to butanol (10/16 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• superpathway of N-acetylneuraminate degradation (13/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Fructose and mannose metabolism
• Glycolysis / Gluconeogenesis
• Inositol phosphate metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.1

Use Curated BLAST to search for 5.3.1.1

Sequence Analysis Tools

See A0A166PXQ0 at UniProt or InterPro

Protein Sequence (286 amino acids)

>Pf1N1B4_4281 Glyceraldehyde-3-phosphate ketol-isomerase (EC 5.3.1.1) (Pseudomonas fluorescens FW300-N1B4)
MRIALDPYMYRNLSLGKMVDKVAELGYEHIELSPREDFLPFYKAPRVDKARIKEFRKALS
DTGVKLSSLLPMYHWAAADEGLRVAAVRNWKRAIQIAVEMDCELVNTEFTGQSDNPLVCE
NQFMRSMDELIPEFEREGIKLDIQAHPYDFCERNNESVDIIRGLDRDWINYLYAAPHTFF
YDDGVGDIASMLKYAGSKLSHLIIADTYNHKASSGLRYIVNPPGVTATVHQHLDIGQGEV
DWEAFFSTLREIKFDGIATVSVFAWEDRPDESNRMMLERVTRELCQ