Protein Info for Pf1N1B4_4219 in Pseudomonas fluorescens FW300-N1B4

Annotation: FIG01074225: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 531 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 56 to 77 (22 residues), see Phobius details amino acids 86 to 105 (20 residues), see Phobius details amino acids 111 to 133 (23 residues), see Phobius details amino acids 144 to 167 (24 residues), see Phobius details amino acids 173 to 194 (22 residues), see Phobius details amino acids 206 to 227 (22 residues), see Phobius details amino acids 239 to 257 (19 residues), see Phobius details amino acids 277 to 304 (28 residues), see Phobius details amino acids 315 to 334 (20 residues), see Phobius details amino acids 341 to 360 (20 residues), see Phobius details amino acids 372 to 395 (24 residues), see Phobius details amino acids 491 to 518 (28 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 19 to 507 (489 residues), 242.6 bits, see alignment E=4.1e-76 PF07690: MFS_1" amino acids 22 to 421 (400 residues), 114.5 bits, see alignment E=2.6e-37

Best Hits

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 79% identity to smk:Sinme_4331)

Predicted SEED Role

"FIG01074225: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z725 at UniProt or InterPro

Protein Sequence (531 amino acids)

>Pf1N1B4_4219 FIG01074225: hypothetical protein (Pseudomonas fluorescens FW300-N1B4)
MSTIASATRSNASATTGVLLMAGIVLATLTEAIASTVLSLGRSDIIGDTYATPDEFAWLD
IGYIALKLIGFMTAPWLMNRFAARHVIISSTLVMGLACGIAAITARLDLLVALRVIQGFA
GGTLLVGGQAIIFRSFPRSHQPILQALFAMGSVVAPATLAPALQGWLIDSQSWAWIFFSV
VPIALAASGLVLIADGPMPAKVRQRPFDWIGFSLISTTLLCFTYVLSQGSRWDWFEEPRI
LWLTVIGAAALLAFLGQQVLAKGQGLLDFSLFKTSDFSFAFIVSFVAGAALFGSAFLIPA
FALSVLGFTPTDAGQLLLPSGAFFVGALLISAYLMQAHRVAPFATVPFGILMIMVAMWML
SGSTSESGADDMMAAILLRGLGLGFLFLSITLIAFSHLNSRNLAGGIGLFNTGRQLGGLI
GVAGLQTLIDHNVVTNAVVLGANVTPGGAALIERMTNTTAMLAVKGMDAAAAGRAATSLL
GRLVTSQSTVIAFDTAFVAVALLFVIAAPILVGIKIGFARYEKVRASRAVA