Protein Info for Pf1N1B4_4194 in Pseudomonas fluorescens FW300-N1B4

Annotation: 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 373 PF00724: Oxidored_FMN" amino acids 5 to 335 (331 residues), 278.4 bits, see alignment E=4.7e-87

Best Hits

KEGG orthology group: K10680, N-ethylmaleimide reductase [EC: 1.-.-.-] (inferred from 90% identity to pba:PSEBR_a2361)

MetaCyc: 54% identical to nitroglycerin reductase (Agrobacterium tumefaciens)
1.7.1.-; 1.7.1.-

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-, 1.3.1.34

Use Curated BLAST to search for 1.-.-.- or 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166PVM5 at UniProt or InterPro

Protein Sequence (373 amino acids)

>Pf1N1B4_4194 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34) (Pseudomonas fluorescens FW300-N1B4)
MSDLNLFTPYTLGALTLSNRVVLAPLTRNRAGKGFVPSEFAAAYYSQRASAGLLISEASQ
ISQQGQGYQDTPGIYTQSQIEGWRKVTDAVHAKGGRIFLQLWHVGRVSHVDLQQQGAAPV
APSALRPATKVFVNNRFEDVSEPRALETDELPGIVNDFRQAAANAIAAGFDGVEIHGANG
YLLDQFIKDGANLRTDAYGGSIANRARLLLEVTAAVAAEIGADRTGIRLSPVSPANGVSS
SDPQAQFDYIVDQLNALDIVYLHVVEGATGGPRDVAPFDFDALRQRFKNTYIANNGYDLD
LATARLTHDKADLIAFGRPFIGNPDLVERLKNGAPLSAFDPSTLYGGGAAGYIDYPTLAE
SSAAANQAQADRA