Protein Info for Pf1N1B4_4165 in Pseudomonas fluorescens FW300-N1B4
Annotation: Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01426, amidase [EC: 3.5.1.4] (inferred from 64% identity to spe:Spro_4710)MetaCyc: 43% identical to linuron hydrolase (Variovorax sp. SRS16)
3.5.1.M21,3.5.1.M19,3.5.1.M20 [EC: 3.5.1.M19, 3.5.1.M20, 3.5.1.M21]
Predicted SEED Role
"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- indole-3-acetate biosynthesis III (bacteria) (2/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamine degradation I (1/1 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
- L-arginine degradation X (arginine monooxygenase pathway) (2/3 steps found)
- acrylonitrile degradation I (1/2 steps found)
- linuron degradation (1/2 steps found)
- superpathway of acrylonitrile degradation (1/3 steps found)
- indole-3-acetate biosynthesis II (5/12 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Aminoacyl-tRNA biosynthesis
- Arginine and proline metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Cyanoamino acid metabolism
- Glutamate metabolism
- Phenylalanine metabolism
- Styrene degradation
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.4, 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 3.5.1.4 or 3.5.1.M19 or 3.5.1.M20 or 3.5.1.M21 or 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166PUR3 at UniProt or InterPro
Protein Sequence (478 amino acids)
>Pf1N1B4_4165 Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7) (Pseudomonas fluorescens FW300-N1B4) MNLNEYVTYDGIGLAQLVATGEVTSAELAELAIAAVQQVNPQINAVIEHWSPTVTEGTGP LAGVPFLIKDLAITSAGRRVELGSRLAQGLVAESDSYLMQRFNAAGLATLGRTTTPEMAF STETESVLQGPTRNPWDTRLSAGGSSGGSGAAVAAGIVPIAHATDAAGSIRVPASYNGLV GLKPTRGRASNGPALDEVFAGFGVQLGLSRTVRDSAALMDIVQGYAPGDPYYTAAPDEGF LAQVGRDPGRLRIGLLDTPWNGAALDPEIAEATLAVARELEALGHRVERVIAPLGTSWDS FVQANAHIWCATLVRWIDGLAAATSRPIDLSTLEPATLACYAYGLEARAVEFAGALEVRN LIARSVAGWFDDFDVLLTPTLPRLPHALDTYSRGAQTMDGLQWTARVFEHSPFTPVFNVA GTPAMSLPLAMSREQGLPIGLQFAARFGAEEVLLRLAGQLEQALPWHERRPQIWVGDD