Protein Info for Pf1N1B4_4048 in Pseudomonas fluorescens FW300-N1B4

Annotation: tRNA-specific 2-thiouridylase MnmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 PF02540: NAD_synthase" amino acids 9 to 83 (75 residues), 24.4 bits, see alignment E=3.8e-09 TIGR00420: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase" amino acids 12 to 364 (353 residues), 468.6 bits, see alignment E=5.6e-145 PF02568: ThiI" amino acids 12 to 81 (70 residues), 22.9 bits, see alignment E=1.5e-08 PF03054: tRNA_Me_trans" amino acids 12 to 207 (196 residues), 273.9 bits, see alignment E=2e-85 PF20259: tRNA_Me_trans_M" amino acids 213 to 280 (68 residues), 82.8 bits, see alignment E=2.5e-27 PF20258: tRNA_Me_trans_C" amino acids 290 to 364 (75 residues), 76 bits, see alignment E=6.1e-25

Best Hits

Swiss-Prot: 97% identical to MNMA_PSEF5: tRNA-specific 2-thiouridylase MnmA (mnmA) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K00566, tRNA-specific 2-thiouridylase [EC: 2.8.1.-] (inferred from 98% identity to pba:PSEBR_a3574)

MetaCyc: 68% identical to tRNA-specific 2-thiouridylase (Escherichia coli K-12 substr. MG1655)
RXN0-2023 [EC: 2.8.1.13]

Predicted SEED Role

"tRNA-specific 2-thiouridylase MnmA"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.-

Use Curated BLAST to search for 2.8.1.- or 2.8.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166PRV7 at UniProt or InterPro

Protein Sequence (377 amino acids)

>Pf1N1B4_4048 tRNA-specific 2-thiouridylase MnmA (Pseudomonas fluorescens FW300-N1B4)
MRDPAPSDTQKKRVIVGMSGGVDSSVSALLLIEQGYEVEGLFMKNWEEDDGTEYCTAMDD
LADAQAVCDKIGIKLHTANFAAEYWDNVFEHFLAEYKAGRTPNPDILCNREIKFKAFLDY
AMMLGADLIATGHYVRRRDIDGRTELLKGLDPNKDQSYFLHAVGGEQIAKTLFPVGELEK
PEVRAIAEKHDLATAKKKDSTGICFIGERRFSDFLKQYLPAQPGEIKTTEGEVIGRHHGL
MYHTIGQRQGLGIGGLKDAGDEPWYVLIKDLEHNELIVGQGNDHPYLFSRALLASDIYWV
NPIDLTEPRKLTAKVRYRQSDQPCTLEKTASGYRATFDDPQRAVTPGQSVVFYDGEICLG
GGVIEVAEPWTSKGQRS