Protein Info for Pf1N1B4_398 in Pseudomonas fluorescens FW300-N1B4

Annotation: Galactonate dehydratase (EC 4.2.1.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 65% identical to DGOD_RALPJ: D-galactonate dehydratase (dgoD) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K01684, galactonate dehydratase [EC: 4.2.1.6] (inferred from 98% identity to pfs:PFLU0032)

MetaCyc: 62% identical to D-galactonate dehydratase (Escherichia coli K-12 substr. MG1655)
Galactonate dehydratase. [EC: 4.2.1.140, 4.2.1.6]

Predicted SEED Role

"Galactonate dehydratase (EC 4.2.1.6)" in subsystem D-galactonate catabolism (EC 4.2.1.6)

MetaCyc Pathways

Entner-Doudoroff pathway III (semi-phosphorylative) (8/9 steps found)
D-galactonate degradation (3/3 steps found)
Entner-Doudoroff pathway II (non-phosphorylative) (7/9 steps found)

KEGG Metabolic Maps

Galactose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.140 or 4.2.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AQR2 at UniProt or InterPro

Protein Sequence (382 amino acids)

>Pf1N1B4_398 Galactonate dehydratase (EC 4.2.1.6) (Pseudomonas fluorescens FW300-N1B4)
MKITKLTTFIVPPRWCFLKVETDEGVTGWGEPVVEGRAHTVAAAVEELSDYLIGKDPRNI
EDIWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGVSVSDLLGGQVRDKIRVYSWI
GGDRPADTARAAKEAVSRGFTAVKMNGTEELQFLDSFEKVDLALANVAAVRDAVGPNVGI
GVDFHGRVHKPMAKVLMKELDPYKLMFIEEPVLSENYEALKELAPLTSTPIALGERLFSR
WDFKRVLSEGYVDIIQPDASHAGGITETRKIANMAEAYDVALALHCPLGPIALAACLQLD
AVCYNAFIQEQSLGIHYNESNDLLDYVKDPRVFDYDKGFVKIPNGPGLGIEINEEYVIER
AAVGHRWRNPIWRHADGSFAEW