Protein Info for Pf1N1B4_392 in Pseudomonas fluorescens FW300-N1B4

Annotation: Electron transfer flavoprotein, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 249 PF01012: ETF" amino acids 30 to 206 (177 residues), 140.4 bits, see alignment E=3e-45

Best Hits

Swiss-Prot: 92% identical to ETFB_PSEAE: Electron transfer flavoprotein subunit beta (etfB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03521, electron transfer flavoprotein beta subunit (inferred from 98% identity to pba:PSEBR_a4019)

Predicted SEED Role

"Electron transfer flavoprotein, beta subunit" in subsystem Acetyl-CoA fermentation to Butyrate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MLB2 at UniProt or InterPro

Protein Sequence (249 amino acids)

>Pf1N1B4_392 Electron transfer flavoprotein, beta subunit (Pseudomonas fluorescens FW300-N1B4)
MKVLVAVKRVVDYNVKVRVKADNSGVDLANVKMSMNPFCEIAVEEAVRLKEKGVATEIVV
VSIGPSTAQEQLRTALALGADRAILVESAEDLTSLAVAKLLKAVVDKEQPQLVILGKQAI
DSDNNQTGQMLAALSGYGQGTFASKVEVSGDSVAVTREIDGGAQTVSLKLPAIVTTDLRL
NEPRYASLPNIMKAKKKPLEVLTPDALGVSTASTNKTVKVEAPAARSAGIKVKSVAELVE
KLKNEAKVI