Protein Info for Pf1N1B4_3916 in Pseudomonas fluorescens FW300-N1B4

Annotation: Transcription-repair coupling factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1149 PF17757: UvrB_inter" amino acids 129 to 217 (89 residues), 95.5 bits, see alignment 6e-31 TIGR00580: transcription-repair coupling factor" amino acids 147 to 1080 (934 residues), 1098.4 bits, see alignment E=0 PF21132: MFD_D3" amino acids 376 to 450 (75 residues), 91.8 bits, see alignment 7.5e-30 PF02559: CarD_TRCF_RID" amino acids 479 to 534 (56 residues), 63.2 bits, see alignment 6.7e-21 PF00270: DEAD" amino acids 604 to 766 (163 residues), 96.3 bits, see alignment E=6.2e-31 PF04851: ResIII" amino acids 605 to 760 (156 residues), 42.6 bits, see alignment E=2.1e-14 PF00271: Helicase_C" amino acids 803 to 908 (106 residues), 65.3 bits, see alignment E=2.1e-21 PF03461: TRCF" amino acids 1008 to 1099 (92 residues), 101.2 bits, see alignment E=1.2e-32

Best Hits

Swiss-Prot: 56% identical to MFD_HAEIN: Transcription-repair-coupling factor (mfd) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Predicted SEED Role

"Transcription-repair coupling factor" in subsystem DNA-replication or Transcription factors bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z6I5 at UniProt or InterPro

Protein Sequence (1149 amino acids)

>Pf1N1B4_3916 Transcription-repair coupling factor (Pseudomonas fluorescens FW300-N1B4)
VPVLRLPLLPAAAGKQHWGNLPGAALSLAIAEAASAAKRFTLLLTADSQSAERLEQELSF
FAPDLPVLHFPDWETLPYDLFSPHQDIISQRISSLYRLPELEHGVLVVPITTALHRLAPT
KFLLGSSLVLDVGQKLDVEQMRTRLEASGYRYVDTVYEHGEFTVRGSLIDLFPMGSKLPF
RIDLFDDEIETLRTFDPENQRSIDKVDTVRLLPAREFPLQKDAVTRFKARFRERFDVDFR
RCPIFQDLSSGITPAGIEYYLPLFFDETSTLFDYLPQDTQVFSLPGIEQAAENFWNDVRN
RYEERRVDPSRPLLPPAELFLPVEDCFARLKSWPRVVASQQDVETGVGRERFPAQPLPNL
AIEAKATQPLAALAGFLDEFPGRVLFTAESAGRREVLLELLERLKLRPKTVDSWPDFVAS
KERLAITIAPLDEGLLLDDPALALVAESPLFGQRVMQRRRREKRADANNDAVIKNLTELR
EGAPVVHIDHGVGRYLGLTILEIDNQAAEFLTLEYAENAKLYVPVANLHLIARYTGSDDA
LAPLHRLGSETWQKAKRKAAEQVRDVAAELLDIYARRAAREGYAFADPKADYATFSAGFP
FEETPDQQTTIEAVRADMLAPKPMDRLVCGDVGFGKTEVAMRAAFIAVHGGRQVAILVPT
TLLAQQHYNSFRDRFADWPVSVEVMSRFKSAKEVNAAVADLAEGKIDIVIGTHKLLQDDV
KIKNLGLVIIDEEHRFGVRQKEQLKALRSEVDILTLTATPIPRTLNMAVSGMRDLSIIAT
PPARRLSVRTFVMEQNKSTVKEALLRELLRGGQVYYLHNDVKTIEKCAADLAELVPEARI
GIGHGQMRERDLEQVMSDFYHKRFNVLIASTIIETGIDVPSANTIIIERADKFGLAQLHQ
LRGRVGRSHHQAYAYLLTPPRQQITPDAEKRLEAIANTQDLGAGFVLATNDLEIRGAGEL
LGDGQSGQIQAVGFTLYMEMLERAVKSIRKGEQPNLDQPLGGGPEVNLRVPALIPEDYLP
DVHARLILYKRIASATDEEGLKDLQVEMIDRFGLLPEPTKNLVRITALKLQAEQLGIKKV
DGGPQGGRIEFATQTPVDPLTLIKLIQSQPKRYKFEGATMFKFQVPMERPEERFNTVEAL
FERLIPTTA