Protein Info for Pf1N1B4_39 in Pseudomonas fluorescens FW300-N1B4

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 856 transmembrane" amino acids 21 to 44 (24 residues), see Phobius details amino acids 255 to 278 (24 residues), see Phobius details PF05228: CHASE4" amino acids 62 to 222 (161 residues), 58.8 bits, see alignment E=1.7e-19 TIGR00229: PAS domain S-box protein" amino acids 303 to 423 (121 residues), 50.1 bits, see alignment E=3e-17 PF00989: PAS" amino acids 307 to 416 (110 residues), 36.4 bits, see alignment E=1.2e-12 PF13426: PAS_9" amino acids 318 to 418 (101 residues), 31.3 bits, see alignment E=5.1e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 426 to 587 (162 residues), 142.2 bits, see alignment E=1.3e-45 PF00990: GGDEF" amino acids 431 to 585 (155 residues), 161 bits, see alignment E=5.2e-51 PF00563: EAL" amino acids 606 to 837 (232 residues), 249.8 bits, see alignment E=6.3e-78

Best Hits

KEGG orthology group: None (inferred from 75% identity to pba:PSEBR_a1797)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161YXL8 at UniProt or InterPro

Protein Sequence (856 amino acids)

>Pf1N1B4_39 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Pseudomonas fluorescens FW300-N1B4)
MDLTAITTAAPARHTPITRNLIFVIGLLFVVGVLIALIALFSIAGRLDAQDLSKTTFYTQ
RALENRITASKNYIVSYANWTTAYDHLNDKVDVQWAYVEQNVGKTLFTIDHYDAVFVIDG
QRTKYATVRGLLVQDQATAYLSTSMATLVDEVQSQESLIEPVSRYTLFEGKPALLTAAAI
VPNDERPAVDPKSTSVLIYVDQLNPEKLATLSADYGLHDLSLTADDVIVPGQPAVALTGT
GYSLVSRLEQPGHQLLWSLLPPLGGALLILALLTAYFFRYVLRTSGHVDASYTSLDLSNQ
ALEASEERFRAVAEAASDWIWETDDQHCITYLSGRFSTVTGFSDQQWLGQNIGQLLNCET
MPLSLWLNKLDEEQAVSHLRCSYRDQSGQQRVCRVSARPIQRKGALLGFRGTASDITDEV
AAHAQIQHLSMHDALTGLPNRNKLARYLEDALALKEQSTALTLLMIDLDNFKPINDSLGH
PAGDAVLLEVAIRLRECTRDIDLVARLGGDEFVLVLHGMDSHAEIDRFCERLLDSLHQPI
HYDHHTLHIGASIGIALSRRQGHLPEELIRCADIALYQAKSNGKKTWCYFAAHMNDQIQH
RRQLESDLRQAIKNNEFVMHYQPRYHVDGKQIVSVEALLRWQHPVQGLLNPDAFIPLAEQ
TDLIVPLGRWVLREACETALTWPGAMMVSVNLSPVQFARSDVIEDVREALIQSRLPASRL
ELEITENVMLIDVDGALATMNALKELGVRLNMDDFGTGYSSLGYLRTYPFDGIKIDKRFI
ASMSNGSNDRAVVQAIINLGKAMGLTVTAEGVETLEQLDFLISDQCHEVQGFYLSRPVDR
HALLPLLKASWDDHPL