Protein Info for Pf1N1B4_3888 in Pseudomonas fluorescens FW300-N1B4
Annotation: Aconitate hydratase (EC 4.2.1.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 85% identical to ACNA_PSEAE: Aconitate hydratase A (acnA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 70% identity to abo:ABO_0694)Predicted SEED Role
"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)
MetaCyc Pathways
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
- partial TCA cycle (obligate autotrophs) (8/8 steps found)
- superpathway of glyoxylate bypass and TCA (10/12 steps found)
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
- ethene biosynthesis V (engineered) (19/25 steps found)
- superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
- TCA cycle I (prokaryotic) (8/10 steps found)
- glyoxylate cycle (5/6 steps found)
- TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
- TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
- TCA cycle VI (Helicobacter) (7/9 steps found)
- TCA cycle VII (acetate-producers) (7/9 steps found)
- mixed acid fermentation (12/16 steps found)
- TCA cycle II (plants and fungi) (6/9 steps found)
- nitrogen remobilization from senescing leaves (5/8 steps found)
- TCA cycle III (animals) (6/10 steps found)
- reductive TCA cycle I (6/11 steps found)
- reductive TCA cycle II (5/12 steps found)
- methylaspartate cycle (9/19 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glyoxylate and dicarboxylate metabolism
- Reductive carboxylate cycle (CO2 fixation)
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.3
Use Curated BLAST to search for 4.2.1.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166PMI5 at UniProt or InterPro
Protein Sequence (913 amino acids)
>Pf1N1B4_3888 Aconitate hydratase (EC 4.2.1.3) (Pseudomonas fluorescens FW300-N1B4) MPSLDSLKTLKTLQVDDKTYHYFSLPEAAKSLGDLDKLPMSLKVLLENLLRWEDEKTVTG ADLKAIAAWLKERRSDREIQYRPARVLMQDFTGVPAVVDLAAMRAAMAKAGGDPQRINPL SPVDLVIDHSVMVDKYASASAFGQNVDIEMQRNHERYAFLRWGQSAFNNFSVVPPGTGIC HQVNLEYLGRTVWTKDEDGRTYAFPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAMLGQ PVSMLIPEVIGFKLTGKLKEGITATDLVLTVTQMLRKKGVVGKFVEFYGDGLADLPLADR ATIANMAPEYGATCGFFPVDEVTLEYLRLSGRTPQTVKLVEAYSKTQGLWRLPGKEPVFT DSLALDMGSVEASLAGPKRPQDRVSLPNVAQAFTDFLGLQFKPSSKEEGRLESEGGGGVA VGNADLIGEADYHHEGSTYRLKNGAVVIAAITSCTNTSNPSVMMAAGLVAKKAVEKGLKR KPWVKSSLAPGSKVVTDYYKAAGLTQYLDELGFALVGYGCTTCIGNSGPLPEPIEKAIQK ADLTVASVLSGNRNFEGRVHPLVKTNWLASPPLVVAYALAGTVRIDISSEPLGNDKDGHP VYLRDIWPSTKEIADAVTQVNTAMFHKEYAEVFAGDEQWQAIEVPQAATYVWNNDSTYIQ HPPFFDDIGGPAPVVKDVEGAKVLALLGDSVTTDHISPAGNIKADSPAGHYLREQGVEPR DFNSYGSRRGNHQVMMRGTFANIRIRNEMLGGEEGGNTIYIPTGEKLPIYDAAMRYQASG TPLVVIAGQEYGTGSSRDWAAKGTNLLGVKAVIAESFERIHRSNLVGMGVLPLQFKLDQN RKSLNLTGKETLDILGLTGVELTPRMNLTLVVTREDGNTEKVEVLCRIDTLNEVEYFKAG GILHYVLRQLIAS