Protein Info for Pf1N1B4_3860 in Pseudomonas fluorescens FW300-N1B4

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 569 TIGR00229: PAS domain S-box protein" amino acids 3 to 122 (120 residues), 56.4 bits, see alignment E=3.4e-19 PF00989: PAS" amino acids 13 to 100 (88 residues), 42 bits, see alignment E=2.9e-14 PF13426: PAS_9" amino acids 13 to 116 (104 residues), 62.4 bits, see alignment E=1.5e-20 PF08448: PAS_4" amino acids 13 to 118 (106 residues), 35.3 bits, see alignment E=4.1e-12 PF08447: PAS_3" amino acids 25 to 110 (86 residues), 50.7 bits, see alignment E=6.2e-17 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 124 to 288 (165 residues), 138.8 bits, see alignment E=1.5e-44 PF00990: GGDEF" amino acids 130 to 285 (156 residues), 153.2 bits, see alignment E=1.9e-48 PF00563: EAL" amino acids 305 to 540 (236 residues), 252.8 bits, see alignment E=1e-78

Best Hits

KEGG orthology group: None (inferred from 80% identity to pba:PSEBR_a1711)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166PM01 at UniProt or InterPro

Protein Sequence (569 amino acids)

>Pf1N1B4_3860 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Pseudomonas fluorescens FW300-N1B4)
MDEKYRRAVDAAAIFSETDLDGRITYVNDQFCAVSGYSREELLGQNHRLLNSGLHSADFF
AAMWRSIALGNIWKGEICNRAKDGSLYWVDSTMVPVLDDATGRVHRYLSIRFDISEKRQL
LQSLQWRVGHDVLTGLPNRAFLSDLLDQALEFSRHENIPLAVCMLDLDGFKAVNDGYGHA
SGDMLLVEVAKRLRDIVRGEDVVARLAGDEFVLVLRYVRDLPELCAALNRVLGAISAPYT
LHGKDINVFASIGVTLFPHDNEDAETLLRHADQAMYVAKQSGRNRFHLFDVSRDQEVKAT
HQTVERVRQALVAGELRLHFQPKVNMRRGVVVGFEALLRWEHPQNGMVPPREFLPLVEDT
DLIIDIGEWVMDQVLSQLHRWQQAGQGWPISINIAARHFQRTDFVDRLKHVLARHAQVAP
QMLDLEIVESVAIENIQHVSACLQACQALGVQFSLGDFGTGYSSLSYLKRLRTQTIKIDK
SFVRDILNDRDDLALTTAVIGLARAFGRQVIAEGLESVEHGELLLRLGCEVAQGYFIARP
MPPGEVPDWVAGFVAPLQWQALEQTAGAL