Protein Info for Pf1N1B4_3853 in Pseudomonas fluorescens FW300-N1B4

Annotation: Xanthine dehydrogenase iron-sulfur subunit (EC 1.17.1.4) / Xanthine dehydrogenase, FAD binding subunit (EC 1.17.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02963: xanthine dehydrogenase, small subunit
  • amino acids 2 to 470 (469 residues), 698.8 bits, E=1.5e-214
• PF00111: Fer2
  • amino acids 17 to 76 (60 residues), 28 bits, E=3.5e-10
• PF01799: Fer2_2
  • amino acids 87 to 158 (72 residues), 97.5 bits, E=7.8e-32
• PF00941: FAD_binding_5
  • amino acids 199 to 362 (164 residues), 179.6 bits, E=9.8e-57
• PF03450: CO_deh_flav_C
  • amino acids 370 to 470 (101 residues), 123.5 bits, E=7.6e-40

Best Hits

KEGG orthology group: K13481, xanthine dehydrogenase small subunit [EC: 1.17.1.4] (inferred from 94% identity to pfo:Pfl01_1797)

Predicted SEED Role

"Xanthine dehydrogenase iron-sulfur subunit (EC 1.17.1.4) / Xanthine dehydrogenase, FAD binding subunit (EC 1.17.1.4)" in subsystem Purine Utilization (EC 1.17.1.4)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• superpathway of purines degradation in plants (14/18 steps found)
• ureide biosynthesis (6/7 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
• theophylline degradation (1/9 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4

Use Curated BLAST to search for 1.17.1.4

Sequence Analysis Tools

See A0A166PLS0 at UniProt or InterPro

Protein Sequence (484 amino acids)

>Pf1N1B4_3853 Xanthine dehydrogenase iron-sulfur subunit (EC 1.17.1.4) / Xanthine dehydrogenase, FAD binding subunit (EC 1.17.1.4) (Pseudomonas fluorescens FW300-N1B4)
VIQFLLNQELRSEHALDPNLTVLNYLREHVGKPGTKEGCASGDCGACTVVVGELQTDDDG
REHIRYRSLNSCLTFVSSLHGKQLISVEDLKHQGELHSVQKAMVECHGSQCGFCTPGFVM
SLFALQKNSDQPDAHKAHEALAGNLCRCTGYRPILAAAEQSCCGKQPDQFDAREAETIAR
LKAIAPTDIGELNSGDKRCLVPLTVADLADLYDAYPQARLLAGGTDLALEVTQFHRTLPV
MIYVGNVAEMKRIERFDDRLEIGAATALSDCYDALKAEYPDFGELLQRFASLQIRNQGTL
GGNIGNASPIGDSPPLLIALGAQIVLCKGETRRTMALEDYFIDYRVTARQESEFIEKIIV
PRASVEQLFRAYKVSKRLDDDISAVCAAFNLRIDTGVIADARVAFGGMAAIPKRAKNCEA
ALLGAPFNNATLERACAALAEDFTPLSDFRASKEYRLLSAQNLLRKYFIELQTPHIETRV
TAYV