Protein Info for Pf1N1B4_3846 in Pseudomonas fluorescens FW300-N1B4
Annotation: D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K07259, D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (penicillin-binding protein 4) [EC: 3.4.16.4 3.4.21.-] (inferred from 94% identity to pba:PSEBR_a1696)Predicted SEED Role
"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)
MetaCyc Pathways
- peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
- peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
- peptidoglycan maturation (meso-diaminopimelate containing) (4/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.4.16.4, 3.4.21.-
Use Curated BLAST to search for 3.4.16.4 or 3.4.21.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (435 amino acids)
>Pf1N1B4_3846 D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) (Pseudomonas fluorescens FW300-N1B4) MIPLNGPGTPTIHNADVSVNPASTMKLVTTYAALEMLGPNHQWKTEFYTDGTLSGGILNG NLYLKGGGDPKLNMEKLWLLMRDLRANGVTQVTGDLVLDRGFFVQPQLPEFNDDGNDDNK PFLVKPDALLVNLKALRFVARNDSGKVLVSVEPPIASIRIDNQVKALNSKQCTGGVRYNP VPQADGSVTVTVGGQLGEGCSSQTYLSLLDHATYTAGAVRAIWKELGGSIQGQDRLAPTP SNAKVLARAYSPDLAEIIRDINKYSNNTMAQQLFLSLGQKFRTDADGDDAKAAQRVVRQW LAKKGITAPHLVMENGSGLSRAERVSAREMAAMLQAAWRSPYSAEFISSMPIAGTDGTMR KRLKTTAMRGEAHVKTGTLNTVRAISGYSRDINGNTWAVVAILNDKAPFGASSVLDQVLL DLYRQPKLPETASVL