Protein Info for Pf1N1B4_3821 in Pseudomonas fluorescens FW300-N1B4

Annotation: 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02333: 2-methylisocitrate dehydratase, Fe/S-dependent
  • amino acids 2 to 858 (857 residues), 1828.4 bits, E=0
• PF00330: Aconitase
  • amino acids 66 to 533 (468 residues), 458.5 bits, E=3.1e-141
• PF00694: Aconitase_C
  • amino acids 656 to 786 (131 residues), 98.3 bits, E=4.5e-32

Best Hits

Swiss-Prot: 84% identical to ACND_SHEON: 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) (acnD) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 86% identity to alv:Alvin_0079)

MetaCyc: 84% identical to 2-methylcitrate dehydratase (Shewanella oneidensis)
RXN-8979 [EC: 4.2.1.117]

Predicted SEED Role

"2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.2.1.79)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• partial TCA cycle (obligate autotrophs) (8/8 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• glyoxylate cycle (5/6 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• mixed acid fermentation (12/16 steps found)
• TCA cycle II (plants and fungi) (6/9 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• TCA cycle III (animals) (6/10 steps found)
• reductive TCA cycle I (6/11 steps found)
• reductive TCA cycle II (5/12 steps found)
• methylaspartate cycle (9/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3, 4.2.1.79

Use Curated BLAST to search for 4.2.1.117 or 4.2.1.3 or 4.2.1.79

Sequence Analysis Tools

See A0A166PKZ1 at UniProt or InterPro

Protein Sequence (864 amino acids)

>Pf1N1B4_3821 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79) (Pseudomonas fluorescens FW300-N1B4)
MNTEFRKPLPGSHLDYFDVRAAVDAISPGAYDTLPYTSRVLAENLVRRCDPATLTESLKQ
FIERKRDLDFPWFPARVVCHDILGQTALVDLAGLRDAIALQGGDPAQVNPVVPTQLIVDH
SLAVERGGFDPEAFEKNRAIEDRRNEDRFHFINWTKKAFKNVDVIPPGNGIMHQINLEKM
SPVIQVRDGVAFPDTCVGTDSHTPHVDALGVIAIGVGGLEAESVMLGRASWMRLPESVGV
ELTGKLQPGITATDMVLALTEYLRKQKVVGAWLEFFGEGASALTLGDRATISNMAPEYGA
TAAMFYIDQQTIDYLKLTGREDEQVQLVESYAKQTGLWADSLKGAQYERGLTFDLSSVVR
NMAGPSNPHARVAVSDLAAKGISGQWDDVPGQMPDGAVIIAAITSCTNTSNPRNVIAAGL
LARNANKLGLTRKPWVKSSLAPGSKTVALYLDEAGLTSELEQLGFGVVAFACTTCNGMSG
ALDPVIQQEIIDRDLYATAVLSGNRNFDGRIHPYAKNAFLASPPLVVAYAIAGTIRFDIE
KDVLGVVDGKEIRLKDIWPSDEEIDAVVKASVKPEQFRQVYIPMFAIHEDTGPKVAPLYD
WREMSTYIRRPPYWEGALAGARPLKGMRPLAVLPDNITTDHLSPSNAIMLDSAAGEYLAK
MGLPEEDFNSYATHRGDHLTAQRATFANPKLFNEMVQENGKVKQGSLARVEPEGQVMRMW
EAIETYMERKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIAAEGFERIHRTNLVGMGV
LPLEFQPGTDRHTLAIDGSETYDVIGDRTPRATLTLVINRKNGERVEVPVTCRLDTAEEV
SIYEAGGVLQRFAQDFLEESAVAV