Protein Info for Pf1N1B4_3766 in Pseudomonas fluorescens FW300-N1B4

Annotation: Uricase (urate oxidase) (EC 1.7.3.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03212: putative urate catabolism protein
  • amino acids 5 to 302 (298 residues), 544.9 bits, E=2e-168
• PF01522: Polysacc_deac_1
  • amino acids 75 to 162 (88 residues), 66 bits, E=1.5e-22

Best Hits

KEGG orthology group: None (inferred from 97% identity to pfl:PFL_4366)

Predicted SEED Role

"Uricase (urate oxidase) (EC 1.7.3.3)" (EC 1.7.3.3)

MetaCyc Pathways

• superpathway of purines degradation in plants (14/18 steps found)
• ureide biosynthesis (6/7 steps found)
• urate conversion to allantoin I (2/3 steps found)

KEGG Metabolic Maps

• Caffeine metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.7.3.3

Use Curated BLAST to search for 1.7.3.3

Sequence Analysis Tools

See A0A161ZAY6 at UniProt or InterPro

Protein Sequence (308 amino acids)

>Pf1N1B4_3766 Uricase (urate oxidase) (EC 1.7.3.3) (Pseudomonas fluorescens FW300-N1B4)
VSADYPRDLIGYGSNPPHPHWPGNARIALSFVLNYEEGGERNILHGDKESEAFLSEMVSA
QPLQGERNMSMESLYEYGSRAGVWRILKLFKEFDIPLTIFAVAMAAQRHPDVIRAMVDAG
HEICSHGYRWIDYQYMDEAQEREHMLEAIRILTEITGERPLGWYTGRTGPNTRRLVMEEG
GFLYDCDTYDDDLPYWEPNNPTGKPHLVIPYTLDTNDMRFTQVQGFNKGDDFFEYLKDAF
DVLYAEGAEAPKMLSIGLHCRLIGRPARLASLKRFIEYAKSHEQVWFSRRVDIARHWHET
HPFQGAAK