Protein Info for Pf1N1B4_3741 in Pseudomonas fluorescens FW300-N1B4

Annotation: Periplasmic protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 704 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details PF17804: TSP_NTD" amino acids 58 to 245 (188 residues), 220 bits, see alignment E=6.4e-69 TIGR00225: C-terminal processing peptidase" amino acids 215 to 571 (357 residues), 319.4 bits, see alignment E=1.3e-99 PF00595: PDZ" amino acids 253 to 332 (80 residues), 32.7 bits, see alignment E=1.6e-11 PF03572: Peptidase_S41" amino acids 376 to 549 (174 residues), 168.5 bits, see alignment E=2e-53 PF11818: DUF3340" amino acids 554 to 695 (142 residues), 134.4 bits, see alignment E=7.7e-43

Best Hits

KEGG orthology group: K03797, carboxyl-terminal processing protease [EC: 3.4.21.102] (inferred from 95% identity to pfo:Pfl01_1679)

Predicted SEED Role

"Periplasmic protease"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.102

Use Curated BLAST to search for 3.4.21.102

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166PIW4 at UniProt or InterPro

Protein Sequence (704 amino acids)

>Pf1N1B4_3741 Periplasmic protease (Pseudomonas fluorescens FW300-N1B4)
MRYQLSLRRISMKHLLPSTALALVIGLGLLPMSSNSFAANSWDKLQPDRDEVIASLNVVE
LLKRHHYSKPPLDDARSVIIYDSYIKLLDPSRSYFMASDIAEFDKWKTQFDDFLKSGDLN
AGFTIYKRYLDRVKARLDFALAELNKGVDKIDFTAKETLLVDRKDAPWLKTTAELDDLWR
KRVKDEVLRMKISGKESKQIQETLTKRYKNQLSRLDQTRAEDIFQAYINTFAMSYDPHTN
YLSPDNAENFDINMSLSLEGIGAVLQSDNDQVKVVRLVPAGPADKTKQVAPADKIIGVAQ
GNKEMVDVVGWRLDEVVKLIRGPKGTVVRLEVIPASNAPNDQTTKIVPITREAVKLEDQA
VKKSVLNLKQDGKDYKLGVIEIPAFYLDFKAFRAGDPDYKSTTRDVKKLLTELQKDKVDG
VVIDLRNNGGGSLQEATELTSLFIDKGPTVLVRNADGRVDVLEDENPGAFYKGPMVLLVN
RLSASASEIFAGAMQDYHRALIIGGQTFGKGTVQTIQPLNHGELKLTLAKFYRVSGQSTQ
HQGVLPDIDYPSIIDTKEIGESALPEAMPWDTIRAAIKPALDPFKPYITQLKSEHDVRTA
KDAEFVFIRDKLALAQKLMSEKTVSLNEAERRAQHADIEAKQLAMENLRRKAKGEEPLKE
LKKEDEDALAGEPDKTKPEDDAYLSETGRILLDYLKLNTAVAKH