Protein Info for Pf1N1B4_3725 in Pseudomonas fluorescens FW300-N1B4

Annotation: Sensory box histidine kinase/response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 transmembrane" amino acids 36 to 55 (20 residues), see Phobius details PF12860: PAS_7" amino acids 186 to 300 (115 residues), 150 bits, see alignment E=6e-48 PF00512: HisKA" amino acids 340 to 407 (68 residues), 51 bits, see alignment E=2.4e-17 PF02518: HATPase_c" amino acids 452 to 560 (109 residues), 83 bits, see alignment E=4.1e-27 PF00072: Response_reg" amino acids 583 to 694 (112 residues), 44.1 bits, see alignment E=4.3e-15

Best Hits

Predicted SEED Role

"Sensory box histidine kinase/response regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (700 amino acids)

>Pf1N1B4_3725 Sensory box histidine kinase/response regulator (Pseudomonas fluorescens FW300-N1B4)
VLPIAAKSLGLPLSSFPGLAWLQGNPLNLPITPLTQGVVLSLAGNFTLFAWVSVLSRTRV
SEHWQAGRFIGQEISARPNARSMLAVQINDLLQLAARFVGEERARQSFIRFAYRQGKGFS
PTQNADGEWIAHTERLLAGVLGASSTRAVVKAAIEGREMQLEDVVRIADETSEVLQFNRA
LLQGAIENITQGISVVDQSLKLVAWNRRYLELFNYPDGLISVGRPIADIIRYNAERGLCG
PGEAQVHVARRLHWMRQGRAHTSERLFPNGRVIELIGNPMPGGGFVMSFTDITAFREAEQ
ALTEANEGLEQRVIERTHELSQLNVALTEAKGTAESANQSKTRFLAAVSHDLMQPLNAAR
LFSAALSHQEENLSSEARKLVHHLDSSLRSAEDLISDLLDISRLENGKINPDRKPFELND
LFHTLGAEFKALAQEQGLKFRVRGSALRVDSDIKLLRRILQNFLTNAFRYAKGPVLLGVR
RRNGELCLEVWDRGPGIPEDKLQVIFEEFKRLDSHQTRAEKGLGLGLAIADGLCRVLGHT
LRVRSWPGRGSVFSVSVPLARAQAATPAKVAELNGHLLSGAQVLCIDNEDSILIGMNSLL
TRWGCQVWTARNREECATLLGDGMRPQLALVDYHLDDGETGTELMAWLRTQLGEPVPGVV
ISADGRPETVAQVHAAGLDYLAKPVKPAALRALLSRYLPL