Protein Info for Pf1N1B4_3711 in Pseudomonas fluorescens FW300-N1B4

Annotation: Cobyric acid synthase (EC 6.3.5.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 497 PF13500: AAA_26" amino acids 17 to 244 (228 residues), 64.5 bits, see alignment E=1.8e-21 TIGR00313: cobyric acid synthase CobQ" amino acids 18 to 489 (472 residues), 482 bits, see alignment E=1.1e-148 PF01656: CbiA" amino acids 18 to 245 (228 residues), 77.1 bits, see alignment E=1.8e-25 PF07685: GATase_3" amino acids 264 to 447 (184 residues), 201.8 bits, see alignment E=1.3e-63

Best Hits

Swiss-Prot: 95% identical to COBQ_PSEPF: Cobyric acid synthase (cobQ) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K02232, adenosylcobyric acid synthase [EC: 6.3.5.10] (inferred from 95% identity to pfo:Pfl01_1646)

MetaCyc: 50% identical to CobQ (Pseudomonas denitrificans (nom. rej.))
Adenosylcobyric acid synthase (glutamine-hydrolyzing). [EC: 6.3.5.10]

Predicted SEED Role

"Cobyric acid synthase (EC 6.3.5.10)" (EC 6.3.5.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161ZEM0 at UniProt or InterPro

Protein Sequence (497 amino acids)

>Pf1N1B4_3711 Cobyric acid synthase (EC 6.3.5.10) (Pseudomonas fluorescens FW300-N1B4)
MTTLKVQGATLMAQGATLMVQGTTSDAGKSTLVTALCRWVTRQGVSVVPFKPQNMALNSA
VTADGGEIGRAQAVQAQAANLEPHTDMNPVLLKPNSDTGAQVIIHGRAVTTMNAVAYHDY
KAIAMQAVLASHERLSAAYPVVMVEGAGSPAEINLRAGDIANMGFAEAVDCPVVLIADIN
RGGVFAHLVGTLELLSPSEQARVKGFIINRFRGDIALLQPGLDWLEERTGKPVIGVLPYV
MDLHLEAEDGIDQRQTDKADQVLKVVVPVLPRISNHTDFDPLRLHPQVDLQFIGPGQAIP
PADLIILPGSKSVRSDLAYLRANGWDTAISRHLRYGGKVLGICGGLQMLGEQVHDPLGLE
GAPGSSAGLGLLAFETQLEEEKQLRNVRGRLALEDAQVSGYEIHAGVTTGPALENAAVQL
DDGRCDGAQSVDGQIFGTYLHGLFESPAACSALLRWAGLQDVQEVDYHALRERDIERLAD
LVEKHLDTGLLRELCGI